PMID: 9525257Apr 3, 1998Paper

Apoptosis induced by fast neutrons versus 60Co gamma-rays in human peripheral blood lymphocytes

International Journal of Radiation Biology
A VralL De Ridder

Abstract

To investigate the effectiveness of high LET fast neutrons compared with low LET 60Co gamma-rays to induce apoptosis in resting lymphocytes. Apoptosis induction was quantified by light microscopic analysis of irradiated lymphocyte samples from healthy donors after 24 h culture. For the dose-response analyses doses ranging from 0.05 to 5 Gy were applied at 1.5 Gy/min (gamma-rays) or 0.2 Gy/min (fast neutrons). To investigate the role of DNA repair in apoptosis induction a dose of 2 Gy was also given at low dose-rate (0.006 Gy/min). Dose-response curves obtained with both radiation qualities were characterized by an initial steep increase in the number of apoptotic cells below 1 Gy, with a flattening of the curves at higher doses towards 5 Gy. The calculated relative biological effectiveness (RBE) values for fast neutrons were close to unity. When a 2 Gy dose was administered at low rather than high dose-rate no decrease in apoptotic cell yield was observed. The dose-response data confirm the high radiosensitivity of lymphocytes and demonstrate that their response to undergo early interphase cell death by apoptosis is largely independent of LET. The observations that apoptosis induction is independent of LET and dose-rate may sug...Continue Reading

Citations

Jan 22, 2011·International Journal of Radiation Biology·Lenka ZarybnickaJan Osterreicher
Oct 14, 2003·International Journal of Radiation Biology·A HolgerssonA E Meijer
Nov 13, 2007·Journal of Veterinary Science·Hae-June Lee, Sung-Ho Kim
Sep 14, 2001·Radiation Research·I Y BelyaevM Harms-Ringdahl
Aug 17, 2002·Canadian Journal of Physiology and Pharmacology·D CoelhoP Bischoff
Nov 2, 2007·Radiation Research·H SchöllnbergerW Hofmann
May 12, 2017·Anais Da Academia Brasileira De Ciências·Neyliane F G Dos SantosAdemir Amaral
May 11, 2000·Journal of Radiation Research·J H Hendry
Apr 25, 2021·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Fredrik KalholmNiels Bassler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis