PMID: 9175263Jan 1, 1996Paper

Apoptosis-induced concomitant release of cytosolic proteins and factors which prevent cell death

Biology of the Cell
M M EndrichH Gehring

Abstract

In the course of the apoptotic cell death, cells fragment into apoptotic bodies, the elimination of which by phagocytosis is thought to avoid the release of cytosolic constituents whose occurrence is indicative for necrotic cell death. Confluent cultures of chicken embryo fibroblasts, however, show a different behaviour. After serum deprivation, they transiently released with the same time course mitogenic activity, lactate dehydrogenase and cytosolic peptidyl prolyl cis-trans isomerases into the serum-free culture medium. The release correlated in time with a decrease of the cell number which started approximately 3 h after serum removal and ceased within approximately 10 h at about half of the initial cell density. Morphological features like cell shrinkage, membrane blebbing and cell fragmentation as well as internucleosomal DNA fragmentation indicated apoptotic cell death whereas necrotic cell death could be excluded. Conditioned medium (M(r) > or = 30 kDa) from serum-deprived cultures of chicken embryo fibroblasts completely prevented chicken embryo fibroblasts to undergo apoptosis as did phorbol 12-myristate, 13-acetate and, to -60%, L-cysteine. Cycloheximide had no effect on serum deprivation-induced apoptosis. From the ...Continue Reading

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Citations

Jun 18, 2002·Ageing Research Reviews·Kurt Heininger
Dec 26, 2008·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov
Nov 19, 2003·Biochemical and Biophysical Research Communications·Daniel GygiHeinz Gehring
Apr 26, 2002·Cell Death and Differentiation·R JemmersonA Treeful

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis