Apoptosis-inducing effect of garcinol is mediated by NF-kappaB signaling in breast cancer cells

Journal of Cellular Biochemistry
Aamir AhmadFazlul H Sarkar

Abstract

Garcinol, obtained from Garcinia indica in tropical regions, is used for its numerous biological effects. Its anti-cancer activity has been suggested but the mechanism of action has not been studied in-detail, especially there is no report on its action against breast cancer cells. Here we tested our hypothesis that garcinol may act as an anti-proliferative and apoptosis-inducing agent against breast cancer cell lines. Using multiple techniques such as MTT, Histone-DNA ELISA, Annexin V-PI staining, Western blot for activated caspases and cleaved PARP, homogenous caspase-3/7 fluorometric assay and EMSA, we investigated the mechanism of apoptosis-inducing effect of garcinol in ER-positive MCF-7 and ER-negative MDA-MB-231 cells. We found that garcinol exhibits dose-dependent cancer cell-specific growth inhibition in both the cell lines with a concomitant induction of apoptosis, and has no effect on non-tumorigenic MCF-10A cells. Our results suggested induction of caspase-mediated apoptosis in highly metastatic MDA-MB-231 cells by garcinol. Down-regulation of NF-kappaB signaling pathway was observed to be the mechanism of apoptosis-induction. Garcinol inhibited constitutive NF-kappaB activity, which was consistent with down-regulat...Continue Reading

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Citations

Jun 30, 2012·Journal of Oncology·Nadia Saadat, Smiti V Gupta
Mar 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zaorui ZhaoKaryn M Frick
Nov 29, 2013·International Journal of Molecular Sciences·Elvira D'Ippolito, Marilena V Iorio
Oct 8, 2015·Journal of Agricultural and Food Chemistry·Yu WangMin-Hsiung Pan
Feb 14, 2014·Journal of Radiation Research·Takahiro OikeTakashi Kohno
Jan 23, 2016·Molecular Nutrition & Food Research·Aamir AhmadFazlul H Sarkar
Jan 31, 2020·Frontiers in Behavioral Neuroscience·Melissa S MonseyJane R Taylor
Jul 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seok KimTaeg Kyu Kwon
Feb 20, 2019·International Journal of Molecular Sciences·Mohd FarhanAamir Ahmad
Apr 18, 2018·Frontiers in Immunology·Xinglong QuShucheng Hua
Dec 21, 2020·Chemical Biology & Drug Design·Bashir AhmadDeog-Hwan Oh
Jun 3, 2021·Cancers·Ruicen HeKarl Riabowol
Feb 21, 2018·Chemical Reviews·Xing-Wei YangGang Xu

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