PMID: 12495814Dec 24, 2002Paper

Apoptosis-inducing neurotoxicity of dopamine and its metabolites via reactive quinone generation in neuroblastoma cells

Biochimica Et Biophysica Acta
M Emdadul HaqueNorio Ogawa

Abstract

Neurotoxic properties of L-dopa and dopamine (DA)-related compounds were assessed in human neuroblastoma SH-SY5Y cells with reference to their structural relationship. L-Dopa and its metabolites containing two free hydroxyl residues on their benzene ring showed toxicity in the cell, which was prevented by superoxide dismutase (SOD) and reduced glutathione (GSH), but not by catalase. Furthermore, a synthetic derivative of DA, 3-hydroxy-4-methoxyphenethylamine (HMPE) containing methoxy residue at position 4 in the benzene ring, exerted partial cytotoxicity, which was not prevented by SOD, GSH or catalase. However, the metabolites containing methoxy residue at position 3 failed to show a toxic effect in the SH-SY5Y cells. Moreover, DA induced apoptotic cell death, which was observed by nuclear and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining and measurement of caspase-3 activity; this compound up-regulated apoptotic factor p53 while down-regulating anti-apoptotic factor Bcl-2. In the cell-free in vitro electron spin resonance (ESR) spectrometry, DA possessing two hydroxyl groups showed generation of DA-semiquinone radicals, which were markedly prevented by addition of SOD or GSH but not by...Continue Reading

References

Nov 1, 1992·Journal of Neurochemistry·Barry Halliwell
Aug 1, 1990·Journal of Neuroscience Research·P P Michel, F Hefti
May 15, 1985·Biochemical Pharmacology·P G Parsons
Sep 15, 1982·Biochemical Pharmacology·Shosuke Ito, K Fujita
Feb 1, 1995·Journal of Neurochemistry·A N BasmaH M Geller
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·T G HastingsM J Zigmond
Sep 28, 1998·Annals of Neurology·E MelamedI Ziv
Dec 3, 1999·Brain Research. Molecular Brain Research·D M Kuhn, R E Arthur
Feb 15, 2000·Annals of the New York Academy of Sciences·S Porat, R Simantov
Mar 8, 2000·Biochemical Pharmacology·B Drukarch, F L van Muiswinkel
Jun 23, 2000·Chemico-biological Interactions·T MiuraK Zhao
Sep 15, 2000·Journal of Neurochemistry·Y HigashiN Ogawa
Dec 15, 2000·Cell·K H Vousden

Citations

Jan 11, 2005·Neurotoxicity Research·Juan Segura Aguilar, Richard M Kostrzewa
Apr 29, 2005·Journal of Chemical Ecology·Mayumi Hachinohe, Hiroshi Matsumoto
Sep 28, 2007·Journal of Chemical Ecology·Mayumi Hachinohe, Hiroshi Matsumoto
Sep 27, 2006·Pituitary·Joseph E Ippolito
May 18, 2005·Biochemical and Biophysical Research Communications·Yutaka MachidaNobutaka Hattori
Jun 27, 2008·The Journal of Biological Chemistry·Yue FuZhongtao Zhang
Nov 30, 2006·International Journal of Toxicology·Challa SureshC S Chetty
Oct 1, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Cheuk-Kwan SunPo-Han Chen
Aug 3, 2012·Expert Review of Neurotherapeutics·Helena Kyunghee Kim, Ana Cristina Andreazza
Jun 25, 2005·The Annals of Thoracic Surgery·Tatiana ZaitsevaAnna Pastuszko
Mar 8, 2005·Drug Metabolism Reviews·Shufeng ZhouYu-Zong Chen
Jun 11, 2004·Journal of Neurochemistry·Youichirou HigashiNorio Ogawa
Dec 3, 2008·The European Journal of Neuroscience·Eva KüppersStephan Grissmer
Oct 12, 2007·Journal of Neuroscience Research·Luciana MoscaMaria d'Erme
Mar 13, 2010·Annals of Neurology·Masato AsanumaMiho Murata
Jun 25, 2015·Carcinogenesis·Sabine A S LangieAndrew R Collins
Aug 8, 2015·The Protein Journal·Masoome KhalifeFrancisco Pan-Montojo
Sep 8, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Gomathi ChanHabsah Abdul Kadir
Mar 13, 2015·Plant Signaling & Behavior·Anderson Ricardo SoaresOsvaldo Ferrarese-Filho
May 22, 2013·TheScientificWorldJournal·Etinosa O IgbinosaOmoruyi G Idemudia
Jun 30, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Milena BorsMaria Koter-Michalak
Dec 18, 2013·Journal of Psychiatric Research·Rafael T de SousaRodrigo Machado-Vieira

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.