Apoptosis induction activity and molecular docking studies of survivin siRNA carried by Fe3O4-PEG-LAC-chitosan-PEI nanoparticles in MCF-7 human breast cancer cells

Journal of Pharmaceutical and Biomedical Analysis
Sanam AramiNasser Samadi

Abstract

Delivery of small interfering RNAs (siRNAs) into cells still remains a challenge in gene delivery studies. Here, we investigated the ability of synthesized Fe3O4-PEG-LAC-chitosan-PEI nanoparticles for siRNA delivery of survivin as the model gene into cells. The cellular uptake of survivin siRNA carried by synthesized nanoparticles into MCF-7 breast cancer cell line was evaluated by florescent microscopy and flowcytometry, both proving the efficacy of nanoparticles in delivery of up to 64.7% in comparison with lipofectamine 2000. Furthermore, the delivery of survivin siRNA by the nanoparticles (nanoplex) induced apoptosis that was assessed through DAPI staining and Annexin V/PI assays. In addition, we evaluated the efficacy of treatment with nanoplexes in the presence of mitoxantrone, as a chemotherapeutic agent. Our data indicated that inhibition of survivin expression increased the cell sensitivity to mitoxantrone. Real-time PCR and western blotting analysis revealed a significant reduction in mRNA and protein levels of survivin upon delivery of siRNA. Molecular docking studies showed that nanoparticles can bind to centeral BIR domain of survivin, exactly above zinc ion location with high affinity (ΔG: -10.3Kcal/mol). Also, th...Continue Reading

References

Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Oct 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tanja Kortemme, David Baker
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Dec 28, 2004·Nature Medicine·Vira BitkoSailen Barik
May 26, 2006·Algorithms for Molecular Biology : AMB·Stephan H BernhartIvo L Hofacker
Nov 14, 2006·Advanced Drug Delivery Reviews·Elias Fattal, Amélie Bochot
Dec 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hu-Lin JiangChong-Su Cho
May 5, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Toshihito TsutsumiHidetoshi Arima
Jul 10, 2007·Cancer Research·Roland H StauberShirley K Knauer
Aug 25, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangBaichao Ma
Mar 20, 2008·Expert Opinion on Therapeutic Targets·Marzia PennatiNadia Zaffaroni
Jun 6, 2008·Gene Therapy·D Schaffert, E Wagner
Jan 21, 2009·Angewandte Chemie·Jens Kurreck
Jan 31, 2009·Nature Reviews. Drug Discovery·Kathryn A WhiteheadDaniel G Anderson
May 9, 2009·Advanced Drug Delivery Reviews·Yu-Kyoung Oh, Tae Gwan Park
Jun 9, 2009·International Journal of Pharmaceutics·Dhananjay JereChong-Su Cho
Jul 2, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Min Sung SuhYu-Kyoung Oh
Jul 14, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sharif HossainToshihiro Akaike
Dec 2, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Amir K VarkouhiGert Storm
May 9, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chunyan WangSubhra Mohapatra
Jan 25, 2013·Molecular Therapy. Nucleic Acids·Christian DohmenErnst Wagner
Nov 7, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Simin SharifiNasser Samadi
Nov 12, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Shan LiuZhong-Gao Gao
Aug 25, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Alexander EweAchim Aigner

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Citations

Oct 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yating SunChenguang Zhou
May 31, 2018·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Fereshteh ShojaeiMilad Khorramian-Ghahfarokhi
Apr 26, 2019·Journal of Cellular Physiology·Yasamin PahlavanAlireza Khabbazi
Sep 1, 2019·International Journal of Molecular Sciences·Keiko YamakawaYoko Matsuda
Dec 8, 2020·Nanotoxicology·Abdallah S AbdelsattarMohamed A Helal
Jan 24, 2021·Carbohydrate Polymers·Zohreh MohammadiMorteza Rafiee Tehrani

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis