Apoptosis induction in cancer cell lines by the carotenoid Fucoxanthinol from Pseudomonas stutzeri JGI 52

Indian Journal of Pharmacology
Megha Shukla, Kilingar Nadumane Varalakshmi

Abstract

Microorganisms produce a variety of pigments and many pigments from bacteria were reported to have therapeutic potential including anticancer effects. The aim of this study is to evaluate the anticancer potential a yellow pigment from newly isolated Pseudomonas stutzeri JGI 52. Serial dilution method was adopted for the isolation of pigmented bacteria from soil sources. Pigment extraction was carried out from bacterial isolates using methanol as the solvent and the pigment was purified by thin layer chromatography. Through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, the effect of the pigment fraction on cancer cells was analyzed. Apoptosis induction was evaluated by caspase-3 activity assay, DNA fragmentation analysis, cell morphology observation by AO-EB staining under the fluorescence microscope, and cellular cytotoxicity was analysed by lactate dehydrogenase (LDH) release assay. Characterization of the purified pigment was by high-performance liquid chromatography and electrospray ionization-mass spectrometry analysis. Significance of the results was confirmed by performing one-way analysis of variance. The pigment (PY3) from P. stutzeri inhibited the proliferation of HeLa, HepG2, and Jurkat cells and...Continue Reading

Citations

Aug 30, 2020·Cancers·Lenka KoklesovaPeter Kubatka
Jul 25, 2019·Frontiers in Oncology·Zhaogang DongYi Zhang

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Datasets Mentioned

BETA
KM386644

Methods Mentioned

BETA
ELISA
caspase assay

Software Mentioned

BLAST
GraphPad prism
GraphPad

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