Apoptosis induction in human breast cancer (MCF-7) cells by a novel venom L-amino acid oxidase (Rusvinoxidase) is independent of its enzymatic activity and is accompanied by caspase-7 activation and reactive oxygen species production

Apoptosis : an International Journal on Programmed Cell Death
Ashis K MukherjeeStephen P Mackessy

Abstract

We report the elucidation of a mechanism of apoptosis induction in breast cancer (MCF-7) cells by an L-amino acid oxidase (LAAO), Rusvinoxidase, purified from the venom of Daboia russelii russelii. Peptide mass fingerprinting analysis of Rusvinoxidase, an acidic monomeric glycoprotein with a mass of ~57 kDa, confirmed its identity as snake venom LAAO. The enzymatic activity of Rusvinoxidase was completely abolished after two cycles of freezing and thawing; however, its cytotoxicity toward MCF-7 cells remained unaffected. Dose- and time-dependent induction of apoptosis by Rusvinoxidase on MCF-7 cells was evident from changes in cell morphology, cell membrane integrity, shrinkage of cells and apoptotic body formation accompanied by DNA fragmentation. Rusvinoxidase induced apoptosis in MCF-7 cells by both the extrinsic (death-receptor) and intrinsic (mitochondrial) signaling pathways. The former pathway of apoptosis operated through activation of caspase-8 that subsequently activated caspase-7 but not caspase-3. Rusvinoxidase-induced intrinsic pathway of apoptosis was accompanied by a time-dependent depolarization of the mitochondrial membrane through the generation of reactive oxygen species, followed by a decrease in cellular gl...Continue Reading

References

Dec 11, 1994·Nucleic Acids Research·M HerrmannJ R Kalden
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Oct 20, 2001·Oncogene·Y LiangN F Schor
Dec 4, 2003·Current Opinion in Cell Biology·Kelly M Boatright, Guy S Salvesen
Mar 20, 2004·Pharmacology & Therapeutics·Amere Subbarao Sreedhar, Peter Csermely
May 25, 2004·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H SeegerA O Mueck
Aug 2, 2005·Archives of Biochemistry and Biophysics·Brett A WagnerC Patrick Burns
Jun 14, 2006·Apoptosis : an International Journal on Programmed Cell Death·Sudharsana Rao AndeElisa Ferrando-May
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Feb 26, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mari SamelEne Siigur
Sep 23, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Raquel Melo AlvesSuely Vilela Sampaio
Jun 23, 2009·Toxicon : Official Journal of the International Society on Toxinology·Fábio L S CostaVeridiana M Rodrigues
Aug 8, 2009·Cell Death and Differentiation·R Franco, J A Cidlowski
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
May 5, 2011·Biochimica Et Biophysica Acta·Gustavo B NaumannEladio F Sanchez
Jul 16, 2011·Chemico-biological Interactions·Glaucio ValdameriMaria E M Rocha
Sep 29, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jamil ZarganHaider A Khan
Nov 23, 2011·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Cynthia RobitailleHude Quan
Dec 6, 2011·Biochemical Pharmacology·Donald S BackosPhilip Reigan
May 5, 2012·Biochimica Et Biophysica Acta·Inês A BarbosaPaulo J Oliveira
May 15, 2012·Toxicon : Official Journal of the International Society on Toxinology·Chunmei GuoMing-Zhong Sun
Apr 5, 2013·Basic & Clinical Pharmacology & Toxicology·Sandra M BurinFabíola A de Castro
Mar 19, 2014·International Journal of Biological Macromolecules·Ashis K MukherjeeSumita Dutta
Jun 19, 2014·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Tássia R CostaSuely V Sampaio

❮ Previous
Next ❯

Citations

Apr 10, 2016·International Journal of Biological Macromolecules·Anthony J SaviolaStephen P Mackessy
Aug 16, 2016·Revista brasileira de hematologia e hemoterapia·João Agostinho Machado-Neto, Fabiola Traina
Jul 11, 2016·International Journal of Biological Macromolecules·Patrícia H RibeiroAndreimar M Soares
Jul 17, 2016·Toxicon : Official Journal of the International Society on Toxinology·Sandra Mara BurinFabíola Attié de Castro
Jan 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Ju SuSheng-Chun Chiu
Feb 8, 2018·Oxidative Medicine and Cellular Longevity·Jignesh LunagariyaShihai Xu
Oct 15, 2018·Journal of Cellular Physiology·Anna LewinskaMaciej Wnuk
Aug 3, 2018·Journal of Cellular Physiology·Ji-Hye SeoJung-Hyun Shim
Jul 5, 2018·Toxins·Stephen P MackessyAnthony J Saviola
Dec 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Flavia Castellano, Valérie Molinier-Frenkel
Nov 27, 2020·International Journal of Biological Macromolecules·Luana Gonçalves BarbosaRenata Santos Rodrigues
Sep 26, 2017·Toxicon : Official Journal of the International Society on Toxinology·Kok Keong TanGopalakrishnakone Ponnampalam
Jun 10, 2021·Applied Microbiology and Biotechnology·Kosuke KasaiTomisato Miura
Apr 9, 2019·Journal of Proteome Research·Brenda Kathryn JonesStephen P Mackessy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.