Apoptosis-like programmed cell death occurs in procambium and ground meristem of pea (Pisum sativum) root tips exposed to sudden flooding

Annals of Botany
D GladishT Niki

Abstract

Pea (Pisum sativum) primary roots form long vascular cavities when grown under wet or flooded conditions at 25 degrees C. It is thought that the cavities are a form of aerenchyma. At 25 degrees C short roots continue to grow after flooding. After roots reach 10 cm long flooding causes rapid cessation of growth, and root tips often become curled. In longer roots the cavities do not extend into the base of the roots, perhaps rendering them ineffective as aerenchyma. It was hypothesized that the resulting growth arrest was due to programmed cell death (PCD) rather than necrosis. Histological examination by light microscope showed that some cells in the primary meristem (elongation) zone of the primary root tips had morphological abnormalities, including misshapen and fragmented nuclei, and cytoplasmic shrinking and fragmentation. Transmission electron microscopy revealed lobing, invagination and chromatin aggregation in nuclei. The affected cells were positive for terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling. Extracted DNA formed a "DNA ladder" during electrophoresis. Cell death usually began in procambium at one or two protoxylem poles and seemed to spread out to nearby tissues, which asymmetrically inhi...Continue Reading

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Citations

Apr 13, 2013·Plant Cell Reports·A KunikowskaA Kaźmierczak
Dec 7, 2007·The Plant Cell·Per MühlenbockStanislaw Karpinski
Dec 5, 2015·Frontiers in Plant Science·Alessandro MattielloLuca Marchiol
Aug 19, 2014·Physiologia Plantarum·Xi-Lu NiWen-Zhe Liu
Jul 1, 2017·Cell Biology International·Elizabeth L KordyumVasyl O Brykov
Jun 10, 2011·Plant Molecular Biology·Yohei NanjoSetsuko Komatsu
Jun 21, 2018·International Journal of Molecular Sciences·Alexander BetekhtinRobert Hasterok
Jan 5, 2014·Molecular Biology Reports·Setsuko KomatsuMohammad Zaman Nouri
Sep 11, 2013·Journal of Proteome Research·Zahed HossainSetsuko Komatsu
Dec 6, 2011·Journal of Proteome Research·Yohei NanjoSetsuko Komatsu

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis