Apoptosis-linked changes in the phosphorylation status and subcellular localization of the spliceosomal autoantigen U1-70K

Cell Death and Differentiation
J DiekerSylviane Muller

Abstract

Apoptosis consists of highly regulated pathways involving post-translational modifications and cleavage of proteins leading to sequential inactivation of the main cellular processes. Here, we focused on the apoptotic processing of one of the essential components of the mRNA splicing machinery, the U1-70K snRNP protein. We found that at an early stage of apoptosis, before the cleavage of the C-terminal part of the protein by caspase-3, the basal phosphorylation of the Ser140 residue located within the RNA recognition motif, increases very significantly. A caspase-dependent, PP1-mediated dephosphorylation of other serine residues takes place in a subset of U1-70K proteins. The U1-70K protein phosphorylated at Ser140 is clustered in heterogeneous ectopic RNP-derived structures, which are finally extruded in apoptotic bodies. The elaborate processing of the spliceosomal U1-70K protein we identified might play an important role in the regulated breakdown of the mRNA splicing machinery during early apoptosis. In addition, these specific changes in the phosphorylation/dephosphorylation balance and the subcellular localization of the U1-70K protein might explain why the region encompassing the Ser140 residue becomes a central autoantig...Continue Reading

References

May 1, 1969·Journal of Ultrastructure Research·W Bernhard
Jan 1, 1996·Annual Review of Biochemistry·A Krämer
Oct 1, 1996·Molecular Biology of the Cell·T Misteli, D L Spector
Aug 1, 1998·The Journal of Biological Chemistry·W Cao, M A Garcia-Blanco
Jan 14, 1999·Genes & Development·M V MurrayA R Krainer
Jul 13, 2000·Cell Death and Differentiation·P J Utz, P Anderson
May 10, 2001·Current Opinion in Cell Biology·C L Will, R Lührmann
Jun 26, 2002·The Journal of Biological Chemistry·Phinikoula S KatsambaIte A Laird-Offringa
Jul 13, 2002·Arthritis and Rheumatism·Eric L GreidingerRobert W Hoffman
Jul 13, 2002·European Journal of Immunology·Alma J NautaAnja Roos
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eric L GreidingerRobert W Hoffman
Mar 26, 2003·Cell Death and Differentiation·U FischerK Schulze-Osthoff
Oct 31, 2003·Biochimie·Alphonse GarciaAngelita Rebollo
Jan 23, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jürgen W C DiekerJo H M Berden
Jul 21, 2004·Rheumatic Diseases Clinics of North America·Ahmed SheriffMartin Herrmann
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fanny MonneauxSylviane Muller
May 29, 2007·Arthritis and Rheumatism·Jürgen W DiekerJohan van der Vlag

❮ Previous
Next ❯

Citations

Sep 29, 2009·PloS One·Helena HernándezCarol V Robinson
Dec 17, 2011·PLoS Pathogens·Guillaume SuffertSébastien Pfeffer
Oct 26, 2010·Apoptosis : an International Journal on Programmed Cell Death·J H FransenJ van der Vlag
Jun 26, 2012·Journal of Autoimmunity·Nicolas SchallSylviane Muller
Jan 8, 2010·Journal of Autoimmunity·Wesley H BrooksYves Renaudineau
Dec 25, 2009·Journal of Autoimmunity·Markus H HoffmannGünter Steiner
Feb 26, 2016·Expert Review of Clinical Pharmacology·Huza ZhangMaria J Cuadrado
Jul 9, 2008·Biochemical Pharmacology·Anna Ivana ScovassiCarlo Pellicciari
Jun 6, 2009·Journal of Internal Medicine·A Rosen, L Casciola-Rosen
May 17, 2011·Annales pharmaceutiques françaises·S Muller
Sep 30, 2014·Wiley Interdisciplinary Reviews. RNA·J Guiro, D O'Reilly
Feb 27, 2015·Autophagy·Christophe MacriSylviane Muller
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Nicole H KattahPaul J Utz
Jun 3, 2017·Autoimmunity Reviews·Sylviane MullerHélène Jeltsch-David
Jan 1, 2013·Genetics & Epigenetics·Marko Radic, Sylviane Muller
Apr 4, 2017·Médecine sciences : M/S·Sylviane Muller
Apr 10, 2018·Frontiers in Aging Neuroscience·Bing Bai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.