Apoptosis mediates decrease in cellularity during the regression of Arthus reaction in cornea

The British Journal of Ophthalmology
N OzakiN Yamanaka

Abstract

The Arthus type allergic reaction is characterised by inflammatory cell infiltration and marked neovascularisation in the cornea. During the healing stages, inflammatory cells and newly formed microvessels gradually disappear. The aim was to establish whether apoptosis affected the regression of inflammatory cells and newly formed microvessels, in order to define more clearly the cellular mechanisms involved in the pathobiology of corneal diseases. Albino male rabbits were injected subcutaneously with 5 mg/ml bovine serum albumin (BSA) incorporated in Freund's complete adjuvant twice weekly. Under the anaesthesia, 30 microl of a 0.5 mg/ml BSA solution was injected into the central corneal stroma to induce an Arthus type allergic reaction. The injured corneas were collected at various time points ranging from 3 to 20 days. Apoptotic cells were identified by both light microscopy using in situ TdT-dUTP nick end labelling (TUNEL) method and electron microscopy. With increasing time after induction of the Arthus reaction, marked neovascularisation and infiltrated inflammatory cells such as polymorphonuclear cells (PMNs) and plasma cells were observed in the cornea. Thereafter, the inflammatory cells and newly formed microvessels gr...Continue Reading

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Citations

Dec 25, 2004·BMC Ophthalmology·Geeta K VemugantiSavitri Sharma
Mar 4, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kazuhide InageYutaka Tamura
Jul 16, 2014·Fish & Shellfish Immunology·Yu-Sheng WuShiu-Nan Chen
Mar 6, 2013·International Journal of Surgery·Sayyed Yazdan AsadiMahmoud Rafieian-Kopaei
Jul 14, 2001·Current Problems in Surgery·M C RobsonM G Franz
Feb 7, 2007·International Review of Cytology·Ian A Darby, Tim D Hewitson

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