Apoptosis of bovine neutrophils during mastitis experimentally induced with Escherichia coli or endotoxin

American Journal of Veterinary Research
Kaat Van OostveldtC Burvenich

Abstract

To determine whether apoptosis of neutrophils was accelerated during mastits experimentally induced by use of Escherichia coli or E coli endotoxin and whether differences were apparent in the response to E coli or endotoxin. 11 healthy lactating Holstein cows. Blood samples were collected from cows at various intervals after intramammary inoculation with E coli or endotoxin. Percentage of apoptotic neutrophils detected after in vitro incubation for 3 hours was determined. Fluorescein isothiocyanate-labeled annexin-V in combination with propidium iodide was used to distinguish apoptosis and necrosis of neutrophils. Total and differential circulating leukocyte counts and rectal temperature were determined at the time of collection of blood samples. Milk yield and milk somatic cell counts were determined at the time of milking. Inoculation of endotoxin did not accelerate in vitro induction of neutrophil apoptosis. However, inoculation of E coli increased the percentage of apoptotic neutrophils. At 18 hours after inoculation, 20% of the neutrophils were apoptotic, compared with 5% before inoculation. Milk somatic cell count and rectal temperature increased, milk production and total leukocyte count decreased, and percentage of imma...Continue Reading

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Citations

Jan 15, 2004·American Journal of Veterinary Research·Sarah A Wagner, Michael D Apley
Jun 6, 2012·The Veterinary Clinics of North America. Food Animal Practice·Kenneth E Leslie, Christina S Petersson-Wolfe
Jan 16, 2010·Veterinary Microbiology·Petra RegenhardHelga Sauerwein
Oct 2, 2013·Veterinary Microbiology·Carme Plumed-FerrerAtte von Wright
Sep 21, 2004·Journal of Dairy Science·E MonfardiniC Burvenich
Jul 25, 2014·The Journal of Veterinary Medical Science·Seiichi HagiwaraHajime Nagahata

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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