PMID: 9523556Apr 2, 1998Paper

Apoptosis of central and peripheral neurons can be prevented with cyclin-dependent kinase/mitogen-activated protein kinase inhibitors

Journal of Neurochemistry
J W MaasP J Kahle

Abstract

Previous studies have indicated that certain members of the cyclin-dependent kinase/mitogen-activated protein kinase superfamily are involved in apoptosis of neuronal cells. Here, we have examined programmed cell death induced by withdrawal of neurotrophic support from CNS (rat retinal) and PNS (chick sympathetic, sensory, and ciliary) neurons. All four neuron types were equally rescued by the purine analogues olomoucine and roscovitine. Olomoucine inhibits multiple cyclin-dependent and mitogen-activated protein kinases with similar potency. Roscovitine is a more selective cyclin-dependent kinase inhibitor; but, so is butyrolactone I, which did not prevent retinal ganglion cell death. The specific p38MAPK inhibitor SB-203580 did not prevent apoptosis in retinal ganglion cells. Death of these cells in the absence of neurotrophic factors was accompanied by morphological changes indicative of apoptosis, including nuclear condensation and fragmentation. Treatment with olomoucine or roscovitine not only prevented these apoptotic changes in retinal ganglion cells but also blocked neurite outgrowth. The survival-promoting activity of olomoucine correlated with its in vitro IC50 for c-Jun N-terminal kinase-1 and its potency to repress ...Continue Reading

Citations

Feb 10, 2000·Annals of the New York Academy of Sciences·N GiocantiV Favaudon
Jan 2, 2001·The Journal of Biological Chemistry·P Z AnastasiadisR A Levine
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brigitte MalgrangePhilippe P Lefebvre
Sep 11, 2003·American Journal of Physiology. Renal Physiology·Peter M PriceJudit Megyesi
Apr 23, 2002·The Journal of Comparative Neurology·Karine LefèvreVincent Castagné
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rachel C SkirrowCaren C Helbing
Sep 18, 2007·Developmental Neurobiology·Judith Pachuau, Miguel Martin-Caraballo
Mar 11, 1999·Current Opinion in Genetics & Development·M D Garrett, A Fattaey
Aug 5, 2000·Progress in Retinal and Eye Research·P Heiduschka, S Thanos
Oct 16, 1999·Brain Research. Molecular Brain Research·C Bossenmeyer-PouriéJ L Daval
Nov 9, 2005·Pharmacology & Therapeutics·Rachael L Neve, Donna L McPhie
Jan 19, 2007·Biochimica Et Biophysica Acta·Lloyd A GreeneSubhas C Biswas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis