Abstract
During development, immature blood vessel networks remodel to form a simplified and efficient vasculature to meet the demand for oxygen and nutrients, and this remodeling process is mainly achieved via the pruning of existing vessels. It has already known that the migration of vascular endothelial cells (ECs) is one of the mechanisms underlying vessel pruning. However, the role of EC apoptosis in vessel pruning remains under debate, especially in the brain. Here, we reported that EC apoptosis makes a significant contribution to vessel pruning in the brain of larval zebrafish. Using in vivo long-term time-lapse confocal imaging of the brain vasculature in zebrafish larvae, we found that EC apoptosis was always accompanied with brain vessel pruning and about 15% of vessel pruning events were resulted from EC apoptosis. In comparison with brain vessels undergoing EC migration-associated pruning, EC apoptosis-accompanied pruned vessels were longer and showed higher probability that the nuclei of neighboring vessels' ECs occupied their both ends. Furthermore, we found that microglia were responsible for the clearance of apoptotic ECs accompanying vessel pruning, though microglia themselves were dispensable for the occurrence of vess...Continue Reading
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Citations
Oct 15, 2018·Cellular and Molecular Life Sciences : CMLS·Rochelle Tixeira, Ivan K H Poon
Jan 29, 2021·Journal of Neuroscience Methods·Shan ZhaoYu Mu
Mar 11, 2021·Cellular and Molecular Life Sciences : CMLS·Timothy J A Chico, Elisabeth C Kugler
Mar 31, 2021·Cellular and Molecular Life Sciences : CMLS·Nathalie Tisch, Carmen Ruiz de Almodóvar
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