Apoptosis of human breast carcinoma cells in the presence of disialosyl gangliosides: II. Treatment of SKBR3 cells with GD3 and GD1b gangliosides

Glycoconjugate Journal
Rui MaSubhash Basu

Abstract

Apoptosis, or programmed cell death, plays an important role in many physiological and diseased conditions. Induction of apoptosis in cancer cells has been monitored during the cells' progression to apoptosis by anti-cancer drugs and inhibitors of the cell surface glycolipids, gangliosides and SA-Le(x) biosyntheses [Basu, S (1991) Glycobiology, 1, 469-475; and ibid, 427-435] in animal tissues and human carcinoma cells, respectively. Induction of apoptosis in cancer cells by cell surface glycolipids in the human breast cancer (SKBR3) cells is the aim in this study. We have employed the disialosyl gangliosides (GD3 and GD1b) to initiate apoptosis in SKBR3 cells grown in culture in the presence of (14)C-L-Serine. At lower concentrations (0-20 microM) of exogenously added non-radioactive GD3, GD1b, or bovine ganglioside mixture (GM1:GD1a:GD1b:GT1a 2:4:4:2), the incorporation of radioactivity in both (14)C-sphingolipid and (14)C-ceramide was higher. However, at higher concentrations (20-100 microM), wherein apoptosis occurred in high frequency, the (14)C-incorporation decreased in both GSLs and ceramide. Apoptosis induction was monitored by the concomitant appearance of caspase-3 activation and the binding of a fluorescent dye PSS-3...Continue Reading

Citations

Mar 25, 2010·Diagnostic Pathology·Sasha GulatiSverre H Torp
May 6, 2016·International Journal of Molecular Sciences·Sun-Hyung HaCheorl-Ho Kim
Dec 18, 2015·JPEN. Journal of Parenteral and Enteral Nutrition·John J MiklavcicM Tom Clandinin
Mar 11, 2008·Glycoconjugate Journal·Shinji HahismotoHiroyuki Kuwano
Jan 31, 2006·Journal of Neurochemistry·H E SaqrA J Yates
Oct 30, 2020·Frontiers in Neuroscience·Simonetta SipioneVaibhavi Kadam

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