Apoptosis of mononuclear cell infiltrates in cardiac allograft biopsy specimens questions studies of biopsy-cultured cells

Transplantation
K C JollowA A Ansari

Abstract

During acute rejection, CD4 and CD8 T cells infiltrate the myocardium and cause myocyte death and dropout. CD4 and CD8 cells use a number of cytotoxic mechanisms, including fas-fas ligand interactions, which lead to apoptotic death. Since fas is expressed on myocytes, we investigated endomyocardial biopsy specimens from cardiac transplant patients to determine whether apoptosis is one of the mechanisms of cell death in acute rejection. Serial sections of individual endomyocardial biopsy specimens from patients histologically diagnosed as having grade 3A rejection (n=22 biopsy specimens), biopsy specimens showing a typical "Quilty effect" (n=10), and specimens with concurrent grade 3A rejection and the Quilty effect (n=6) were evaluated using the C-terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) technique for frequency of apoptosis in myocytes and mononuclear cell infiltrates. None of the examined sections showed detectable evidence of apoptotic myocytes, even within regions clearly showing myocyte damage. Of interest was our consistent finding that 85-98% of mononuclear cell infiltrates within biopsy specimens scored as having grade 3A rejection had undergone apoptosis. In marked contrast, 9...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A D DubyD A Fox
Nov 24, 1995·Science·Z XiaM E Greenberg
Feb 2, 1995·Nature·J DheinP H Krammer
Mar 10, 1995·Science·S Nagata, P Golstein
Oct 19, 1995·Nature·D BellgrauR C Duke
Jun 1, 1995·Experimental Cell Research·R J GrandC D Gregory
Dec 1, 1994·Immunology Today·G NúñezM González-García
Jul 1, 1994·Cancer Immunology, Immunotherapy : CII·T L Whiteside, G Parmiani
Feb 11, 1994·Science·V GagliardiniJ Yuan
Jul 1, 1993·Immunology Today·D KabelitzK Pechhold
Oct 17, 1996·The New England Journal of Medicine·J NarulaB A Khaw

❮ Previous
Next ❯

Citations

May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·F G BlankenbergH W Strauss
Feb 15, 2000·International Reviews of Immunology·O M Martinez, S M Krams
Sep 2, 2010·Revista española de cardiología·Carmen CristóbalJuan C Martínez
Dec 10, 1998·The American Journal of Pathology·E Van HoffenR A De Weger
Feb 3, 2005·Transplantation Proceedings·H A de Groot-KrusemanW Weimar
Nov 7, 1998·The Journal of Thoracic and Cardiovascular Surgery·P W VriensR C Robbins
Jul 13, 2005·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Kimberly E ChuCharles C Marboe
Jan 15, 2002·Cardiology Clinics·L W MillerB M McManus
May 16, 1998·Transplantation·D Kabelitz
May 26, 2001·Transplantation·B XuC D Platsoucas
Jan 26, 2010·Transplantation·Seyedhossein AharinejadMichael Grimm
Jul 10, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M H KownR C Robbins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis