Apoptosis of terminally differentiated chondrocytes in culture

Experimental Cell Research
G GibsonM Roque

Abstract

During the process of endochondral ossification chondrocytes progress through stages of terminal differentiation culminating in apoptotic death. We have developed a serum-free suspension culture that allows terminal differentiation and facilitates the investigation of factors affecting chondrocyte apoptosis. We have found that chondrocytes not committed to terminal differentiation, i.e., those from the caudal region of chick embryo sterna, a region that remains cartilaginous for some months after the chick hatches, maintained high viability in serum-free suspension culture. A strong dependence of viability on culture density and sensitivity to induction of apoptosis with the protein kinase inhibitor, staurosporine, was consistent with the proposal that these chondrocytes, like nearly all cells, require intercellular communication for survival. Chondrocytes that were committed to terminal differentiation, i.e., those from the cephalic region of chick embryo sterna, a region that is replaced by bone before the chick hatches, expressed the hypertrophic phenotype but maintained their viability in culture for only approximately 6 days. Subsequent cell death was very consistent between cultures and shown to occur by an apoptotic proc...Continue Reading

References

Nov 1, 1991·Bone and Mineral·J A HoylandA J Freemont
Oct 1, 1991·American Journal of Reproductive Immunology : AJRI·I Leibovitch, R Buttyan
Jan 1, 1989·Connective Tissue Research·J F GuoD K MacCallum
Nov 1, 1989·The Journal of Cell Biology·P BrucknerM A Spycher
Aug 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G J GibsonM B Schaffler
Mar 1, 1995·Osteoarthritis and Cartilage·A E Sams, A J Nixon
Jul 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G J GibsonJ H Kimura
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M KozopasR W Craig
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E R LeeJ S Mort
Jan 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G D WalkerT R Oegema
Feb 10, 1995·Cell·A ErlebacherR Derynck
Dec 1, 1994·Current Opinion in Cell Biology·G P Linette, S J Korsmeyer
Aug 1, 1994·The Journal of Cell Biology·Y IshizakiM C Raff
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T AignerK von der Mark
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Z ZhangL C Andersson
May 1, 1996·The Journal of Bone and Joint Surgery. American Volume·E B Hunziker, L C Rosenberg
Jun 1, 1996·The Journal of Cell Biology·M WeilM C Raff
Dec 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Y MullerJ Clos
Jan 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M AliniA R Poole
Sep 1, 1996·Clinical Orthopaedics and Related Research·M Butnariu-EphratZ Nevo
Jan 1, 1995·European Journal of Human Genetics : EJHG·W A Horton
Aug 1, 1960·The Journal of Bone and Joint Surgery. British Volume·J Trueta, V P Amato

❮ Previous
Next ❯

Citations

Nov 23, 2000·Annals of the Rheumatic Diseases·F HéraudM F Harmand
Apr 5, 2000·Molecular Pathology : MP·M O'DonovanE F Gaffney
Oct 14, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Jian-Chun JiangYong-Jun Wang
Aug 11, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M E NuttallM W Lark
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G GibsonL Zhang
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H I Roach, N M Clarke
Feb 15, 2000·Progress in Histochemistry and Cytochemistry·G I Malinin, T I Malinin
Sep 30, 2005·Journal of Anatomy·M ColittiJ S Price
Aug 22, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yoshitaka YamanakaYoshiki Seino
May 4, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zhi-Yong LiChuan-Sen Zhang
Dec 4, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Erik Kern HarringtonKathy K H Svoboda
Mar 27, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·K McQueeney, C N Dealy
Mar 1, 2002·Osteoarthritis and Cartilage·S HashimotoM Lotz
Mar 12, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Sara Hosseini-FarahabadiJoy M Richman
Mar 12, 1999·Mechanisms of Development·I S KimS Mundlos
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M S LeeC C Chao
Sep 20, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T AizawaL C Gerstenfeld
Jan 22, 2000·Arthritis and Rheumatism·S R TewC W Archer
May 6, 1999·Journal of Cellular Physiology·K MansfieldI M Shapiro
Aug 3, 1999·Journal of Cellular Physiology·K KühnM Lotz
Nov 21, 1998·Microscopy Research and Technique·K K Svoboda
Nov 21, 1998·Microscopy Research and Technique·G Gibson
Dec 25, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Christopher S Adams, Irving M Shapiro
Dec 18, 2008·Cell and Tissue Research·Sonja GaberAnnelie-Martina Weinberg
Dec 2, 2009·Apoptosis : an International Journal on Programmed Cell Death·Chien-Yuan WangShih-Chieh Hung
Jul 16, 1999·Journal of Orthopaedic Trauma·M L OlmedoA A Marino
Aug 15, 2001·American Journal of Physiology. Cell Physiology·C C TeixeiraI M Shapiro
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K KühnM Lotz
Oct 12, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·L CaoB B Yang
Dec 5, 1998·Biochimica Et Biophysica Acta·S B Shears
Sep 17, 2021·Endocrine·Miriam E A TschaffonMelanie Haffner-Luntzer

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