Apoptosis related to steroid sulfatase at the cone-shaped projection in nephron morphogenesis

Anatomical Science International
Megumi NishikawaTaichi Ezaki

Abstract

Steroid sulfatase (STS) is a microsomal enzyme catalysing the hydrolysis of steroid 3beta-sulfates, including estrogen sulfate, at organ target sites. Steroid sulfatase activity, as well as the activity of various estrogens, has been reported to influence the development of organs. In the kidney, progenitor cells of nephrons develop from mesenchymal cells and finally attach to the ureteric duct at the region termed the cone-shaped projection. However, the mechanisms of this process have not been fully investigated. In the present study, STS-related apoptosis occurring between the renal vesicle and ureteric duct was examined using immunohistochemical techniques. Immunoreactivity to STS was demonstrated at the cortical metanephric mesenchyme surrounding new vessels and was pronounced at the junction between the renal vesicle and ureteric duct (cone-shaped junction). Steroid sulfatase-transfected LLC-RK1 cells (rat proximal cell line) were also examined in vitro. Apoptosis occurred when an estrogen precursor (ELS) was added at concentrations between 10(-4) and 10(-2) mol/L. These results indicate that STS is synthesized in the progenitor cells of proximal tubules. Moreover, STS may be related to apoptosis occurring at the junction...Continue Reading

References

Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A BallabioG Romeo
May 16, 1985·Biochemical and Biophysical Research Communications·J P SimardP L Chang
May 1, 1973·Journal of Neurochemistry·Y Kishimoto
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W E StumpfY Tanaka
May 20, 2003·Kidney International·Zoia B LevashovaAlan O Perantoni
Dec 6, 2003·American Journal of Physiology. Renal Physiology·Dylan L Steer, Sanjay K Nigam
Dec 16, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Vincenzo PezziBruce R Carr
Mar 3, 2004·Journal of Biochemical and Molecular Toxicology·Takahiro KusumegiHideko Sone

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