Apoptosis signal-regulating kinase 1 inhibition in in vivo and in vitro models of pulmonary hypertension.

Pulmonary Circulation
Kathryn S WilsonDavid J Welsh

Abstract

Pulmonary arterial hypertension, group 1 of the pulmonary hypertension disease family, involves pulmonary vascular remodelling, right ventricular dysfunction and cardiac failure. Oxidative stress, through activation of mitogen-activated protein kinases is implicated in these changes. Inhibition of apoptosis signal-regulating kinase 1, an apical mitogen-activated protein kinase, prevented pulmonary arterial hypertension developing in rodent models. Here, we investigate apoptosis signal-regulating kinase 1 in pulmonary arterial hypertension by examining the impact that its inhibition has on the molecular and cellular signalling in established disease. Apoptosis signal-regulating kinase 1 inhibition was investigated in in vivo pulmonary arterial hypertension and in vitro pulmonary hypertension models. In the in vivo model, male Sprague Dawley rats received a single subcutaneous injection of Sugen SU5416 (20 mg/kg) prior to two weeks of hypobaric hypoxia (380 mmHg) followed by three weeks normoxia (Sugen/hypoxic), then animals were either maintained for three weeks on control chow or one containing apoptosis signal-regulating kinase 1 inhibitor (100 mg/kg/day). Cardiovascular measurements were carried out. In the in vitro model, pr...Continue Reading

References

Sep 10, 1998·American Journal of Respiratory and Critical Care Medicine·P H ScottR Plevin
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Taraseviciene-StewartR M Tuder
Apr 3, 2001·The Journal of Biological Chemistry·K Hirota, G L Semenza
Oct 5, 2001·Free Radical Biology & Medicine·E MinetC Michiels
Dec 26, 2001·Pharmacology & Therapeutics·T K Jeffery, J C Wanstall
Jun 6, 2003·The Journal of Biological Chemistry·Maija HirsiläJohanna Myllyharju
Aug 5, 2003·BioFactors·Martine Torres, Henry Jay Forman
Jan 1, 2004·American Journal of Respiratory and Critical Care Medicine·Rebecca BowersNorbert F Voelkel
Jan 22, 2004·Circulation·Rohit BudhirajaPaul M Hassoun
Jan 23, 2004·Cardiovascular Research·Nazzareno GaliéAngelo Branzi
Mar 27, 2004·The Journal of Biological Chemistry·Shi-wen XuDavid J Abraham
Dec 14, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Joerg HerrmannAmir Lerman
May 17, 2005·Experimental Physiology·John C McGrathCraig J Daly
Nov 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Seok J KwonYong J Lee
Sep 22, 2006·Antioxidants & Redox Signaling·James A McCubreyRichard A Franklin
Oct 24, 2006·Pulmonary Pharmacology & Therapeutics·Heather J MortimerDavid J Welsh
Jun 19, 2007·European Journal of Internal Medicine·Rahman Shah
Aug 2, 2007·The European Respiratory Journal·O PakA Peacock
Feb 14, 2008·Advances in Experimental Medicine and Biology·Eva Nozik-Grayck, Kurt R Stenmark
Jan 7, 2010·Journal of Hypertension·Patrick Crosswhite, Zhongjie Sun
Dec 17, 2010·World Journal of Cardiology·Vincent G DemarcoKevin C Dellsperger
Dec 24, 2010·British Journal of Pharmacology·Reshma S BaligaAdrian J Hobbs
Mar 23, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dongmin ShaoStephen J Wort
Oct 13, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Ryoichi HayakawaHidenori Ichijo
Dec 4, 2012·Free Radical Biology & Medicine·Olga RafikovaStevan P Tofovic
Jan 19, 2013·Therapeutic Advances in Respiratory Disease·Véronique Freund-MichelBernard Muller
Jun 26, 2013·High Altitude Medicine & Biology·David J Welsh, Andrew J Peacock
Jul 16, 2013·Methods in Enzymology·Yong SonHyun-Ock Pae
Jul 31, 2013·Comprehensive Physiology·Saurabh AggarwalStephen M Black
Aug 3, 2013·Free Radical Research·A M TormosJ Sastre
Feb 5, 2014·Biochimica Et Biophysica Acta·Rita Nogueira-FerreiraTiago Henriques-Coelho
May 6, 2014·The European Respiratory Journal·Michiel Alexander de RaafHarm Jan Bogaard
Jun 22, 2014·Circulation Research·Yen-Chun LaiMark T Gladwin

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISAs
ELISA

Clinical Trials Mentioned

NCT02234141

Software Mentioned

ImageJ

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