Apoptotic and chemotherapeutic properties of iron (III)-salophene in an ovarian cancer animal model.

Drug Design, Development and Therapy
Thilo S LangeLaurent Brard

Abstract

The cytotoxicity of organometallic compounds iron(III)-, cobalt(III)-, manganese(II)-, and copper(II)-salophene (-SP) on platinum-resistant ovarian cancer cell lines was compared. Fe-SP displayed selective cytotoxicity (IC(50) at ~1 muM) against SKOV-3 and OVCAR-3 cell lines while Co-SP caused cytotoxic effects only at higher concentrations (IC(50) at 60 muM) and Cu-SP effects were negligible. High cytotoxicity of Mn-SP (30-60 muM) appeared to be nonspecific because the Mn-chloride salt reduced cell viability similarly. The effect of Fe-SP at 1 muM proved to be ovarian cancer cell selective when compared to a panel of cell lines derived from different tumors. The first irreversible step in the induction of cell death by Fe-SP occurred after 3 hrs as indicated by the mitochondrial transmembrane potential (DeltaPsim) and was mainly linked to apoptotic, not necrotic events. To evaluate the toxicity of Fe-SP in vivo we conducted an acute toxicity study in rats. The LD(50) of Fe-SP is >2000 mg/kg orally and >5.5 mg/kg body weight by intraperitoneal injection. An ovarian cancer animal model showed that the chemotherapeutic relevant dose of Fe-SP in rats is 0.5-1 mg/kg body weight. The present report suggests that Fe-SP is a potential...Continue Reading

References

Jan 1, 1994·European Journal of Clinical Pharmacology·P Köpf-Maier
Sep 1, 1996·American Journal of Obstetrics and Gynecology·G S RoseJ C Hiserodt
Dec 22, 1999·The Journal of Clinical Investigation·G I Shapiro, J W Harper
Oct 3, 2003·Cancer Cell·Andrew B Gladden, J Alan Diehl
Oct 8, 2003·Gynecologic Oncology·Mario M LeitaoDavid R Spriggs
Sep 14, 2004·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H E LambertG J S Rustin
Mar 15, 2005·Biochemical Pharmacology·Ruili HuangDavid G Covell
Nov 2, 2005·The Journal of Pathology·R KimK Arihiro
Jan 9, 2008·Journal of Inorganic Biochemistry·Getachew A Woldemariam, Subhrangsu S Mandal

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