PMID: 18203492Jan 22, 2008Paper

Apoptotic and necrotic influence of dental resin polymerization initiators in human gingival fibroblast cultures

Dental Materials Journal
Kouhei MasukiMasayuki Okazaki

Abstract

The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators--namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)--on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 microM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases). All four dental resin polymerization initiators induced G0/G1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.

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Citations

Jan 29, 2013·Journal of Materials Science. Materials in Medicine·F J Rodríguez-LozanoJ M Moraleda
Aug 7, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Mohamed ShehataFranz X Reichl
Dec 21, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Joachim VolkWerner Geurtsen
Oct 30, 2009·International Journal of Molecular Sciences·Athina BakopoulouPavlos Garefis
Sep 30, 2008·Journal of Dentistry·Ryo NishikioriMasayuki Okazaki
Mar 11, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·Adilson Y FuruseDavid C Watts
Apr 27, 2017·European Journal of Oral Sciences·Bo W NilsenVibeke B Michelsen
Mar 5, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Dragica ManojlovicBiljana Nikolić
Dec 17, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoshinori Kadoma, Seiichiro Fujisawa
Jun 9, 2012·Dental Update·Luke J RickmanJulian D Satterthwaite
Jan 27, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·J VolkW Geurtsen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis