Apoptotic cell death induced by hydrogen peroxide in NT2 parental and mitochondrial DNA depleted cells

Neurochemistry International
Sandra M CardosoCatarina R Oliveira

Abstract

Oxidative stress has been implicated in several pathologies associated with degenerative processes. Mitochondria are involved in cell death by necrosis or apoptosis due to a large load of Ca2+, the formation of reactive oxygen species (ROS), mitochondrial depolarization and the release of cytochrome c that initiates the caspase cascade. Nevertheless, the role of mitochondria in cell death processes induced by hydrogen peroxide (H2O2) has not been fully established. In this study, we analyzed the cytotoxic effect of H2O2 on rho+ human teratocarcinoma (NT2) cells and on mitochondria-DNA depleted rho0 NT2 cells, lacking functional mitochondria. The cells were exposed to H2O2 for 24 h and cell viability was dose-dependently decreased in both cell lines upon H2O2 exposure, although cell susceptibility was higher in rho0 NT2 cells. Moreover a decrease in mitochondrial membrane potential (Deltapsi(m)), mitochondrial cytochrome c release, caspases activation and DNA fragmentation were largely induced by H2O2 and occurred in both cell lines. Nevertheless, increased cell toxicity in rho0 cells upon H2O2 exposure was accompanied by a higher activation of the effector caspases-3 and -6. The data support that, in general, no differences wer...Continue Reading

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Citations

May 10, 2005·DNA Repair·Bennett Van HoutenJanine H Santos
Jan 16, 2007·The Journal of Investigative Dermatology·Danielle Paixão-CavalcanteDenise V Tambourgi
Jan 19, 2006·The Journal of Investigative Dermatology·Danielle Paixão-CavalcanteDenise V Tambourgi
Nov 15, 2014·Molecular Neurobiology·A R EstevesS M Cardoso
Nov 15, 2005·British Journal of Pharmacology·Uta KeilWalter E Müller
Sep 15, 2014·Molecular Neurobiology·Daniel SantosSandra M Cardoso
Oct 15, 2013·Biochimica Et Biophysica Acta·A R EstevesS M Cardoso
Nov 1, 2011·The International Journal of Biochemistry & Cell Biology·Guillaume RommelaerePatricia Renard
Aug 26, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Susana M CardosoA Cristina Rego
Jul 20, 2016·Pharmacology Research & Perspectives·Ayeshah A RosdahShiang Y Lim
Nov 12, 2010·American Journal of Physiology. Cell Physiology·Guillaume RommelaereThierry Arnould
May 28, 2019·BioMedicine·Ya-Chen YangZhi-Hong Wang

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