Abstract
Oxidative stress has been implicated in several pathologies associated with degenerative processes. Mitochondria are involved in cell death by necrosis or apoptosis due to a large load of Ca2+, the formation of reactive oxygen species (ROS), mitochondrial depolarization and the release of cytochrome c that initiates the caspase cascade. Nevertheless, the role of mitochondria in cell death processes induced by hydrogen peroxide (H2O2) has not been fully established. In this study, we analyzed the cytotoxic effect of H2O2 on rho+ human teratocarcinoma (NT2) cells and on mitochondria-DNA depleted rho0 NT2 cells, lacking functional mitochondria. The cells were exposed to H2O2 for 24 h and cell viability was dose-dependently decreased in both cell lines upon H2O2 exposure, although cell susceptibility was higher in rho0 NT2 cells. Moreover a decrease in mitochondrial membrane potential (Deltapsi(m)), mitochondrial cytochrome c release, caspases activation and DNA fragmentation were largely induced by H2O2 and occurred in both cell lines. Nevertheless, increased cell toxicity in rho0 cells upon H2O2 exposure was accompanied by a higher activation of the effector caspases-3 and -6. The data support that, in general, no differences wer...Continue Reading
References
Oct 15, 1979·The Biochemical Journal·D LloydB Chance
Nov 1, 1992·Journal of Neurochemistry·B Halliwell
Jan 1, 1989·Free Radical Biology & Medicine·U RafalowskaR A Floyd
Jul 1, 1973·The Biochemical Journal·A Boveris, B Chance
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jul 1, 1995·Free Radical Biology & Medicine·G Benzi, A Moretti
Feb 1, 1996·Journal of Pharmacological and Toxicological Methods·C M PalmeiraK B Wallace
Aug 15, 1997·The Biochemical Journal·G M Cohen
Oct 27, 1997·Neurology·R H SwerdlowW D Parker
Aug 7, 1998·Neuroreport·A M GormanS Ceccatelli
Feb 16, 1999·Oncogene·A DumontM L Schmitz
Oct 9, 1999·The Journal of Biological Chemistry·S JiangD P Jones
Jun 6, 2000·The Journal of Biological Chemistry·S ZhuangI E Kochevar
Oct 24, 2000·Cell Death and Differentiation·M SawadaY Nozawa
Oct 10, 2001·The Journal of Cell Biology·A FortinR S Slack
Jan 12, 2002·The Journal of Biological Chemistry·Hiroki AokiSeigo Izumo
Feb 22, 2002·Experimental Cell Research·N TakeyamaT Tanaka
Mar 19, 2002·Brain Research·Sandra Morais CardosoCatarina R Oliveira
Apr 19, 2002·Biochemical Pharmacology·Tal Gruss-Fischer, Ina Fabian
Nov 8, 2002·Neurochemistry International·H Bai, G W Konat
Nov 8, 2002·Neuroscience·R A KirklandJ L Franklin
Jan 3, 2003·Neurochemistry International·Laszlo TretterVera Adam-Vizi
Apr 12, 2003·Free Radical Research·Sandra M Cardoso, Catarina R Oliveira
Aug 5, 2003·Neuroscience·E MilushevaE S Vizi
Citations
May 10, 2005·DNA Repair·Bennett Van HoutenJanine H Santos
Dec 17, 2014·Molecular Neurobiology·Pradip K KamatNeetu Tyagi
Jan 16, 2007·The Journal of Investigative Dermatology·Danielle Paixão-CavalcanteDenise V Tambourgi
Jan 19, 2006·The Journal of Investigative Dermatology·Danielle Paixão-CavalcanteDenise V Tambourgi
Nov 3, 2005·Neurochemistry International·G FerrettiL Provinciali
Nov 15, 2014·Molecular Neurobiology·A R EstevesS M Cardoso
Nov 15, 2005·British Journal of Pharmacology·Uta KeilWalter E Müller
May 26, 2015·Biochimica Et Biophysica Acta·Maricarmen CristofaroGaetano Villani
Sep 15, 2014·Molecular Neurobiology·Daniel SantosSandra M Cardoso
Oct 15, 2013·Biochimica Et Biophysica Acta·A R EstevesS M Cardoso
Dec 22, 2005·Biochimica Et Biophysica Acta·Tsung-I PengMei-Jie Jou
Nov 1, 2011·The International Journal of Biochemistry & Cell Biology·Guillaume RommelaerePatricia Renard
Aug 26, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Susana M CardosoA Cristina Rego
Jul 20, 2016·Pharmacology Research & Perspectives·Ayeshah A RosdahShiang Y Lim
Apr 11, 2017·Oncogenesis·H-Y TsengH-L Hsu
Sep 24, 2005·Journal of Cellular Biochemistry·Hammer B ChenSynthia H Sun
Nov 12, 2010·American Journal of Physiology. Cell Physiology·Guillaume RommelaereThierry Arnould
May 28, 2019·BioMedicine·Ya-Chen YangZhi-Hong Wang
Apr 9, 2014·Food & Function·Chun-Lin LiuMei-Chin Yin
Jun 17, 2005·Cancer Research·Shuli XiaJohn Laterra