Apoptotic cell therapy for cytokine storm associated with acute severe sepsis.

Cell Death & Disease
Netanel KarbianDror Mevorach

Abstract

Sepsis has no proven pharmacologic treatment other than appropriate antibiotic agents, fluids, vasopressors as needed, and possibly corticosteroids. It is generally initiated mainly by the simultaneous recognition by various components of the innate immune system of either pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). In the current study, we employed the murine cecal ligation and puncture (CLP) model for sepsis to evaluate the effect of post-CLP infusion of apoptotic cells (Allocetra-OTS) on a CLP severe sepsis model. Cardiovascular evaluation, acute kidney injury (AKI), acute liver injury (ALI), and hematological and metabolic function were evaluated. Cytokine and chemokine profiles were measured by Multiplex ELISA and mitochondrial function, and glycolysis by Seahorse. The Murine Sepsis Score (MSS) was used for disease severity definition. CLP mice had low blood pressure, poor cardiac output, and lung dysfunction, as well as AKI, ALI, and thrombocytopenia, which correlated with the MSS and corresponded to a cytokine/chemokine storm. Apoptotic cell administration markedly improved the cytokine and chemokine storm and restored the impaired mitochondrial and glycolytic function ...Continue Reading

References

Oct 1, 1995·Critical Care Medicine·J C MarshallW J Sibbald
Feb 14, 2004·Intensive Care Medicine·Simon FinferJohn Myburgh
Mar 19, 2005·Critical Care : the Official Journal of the Critical Care Forum·Greg S MartinMarc Moss
Aug 10, 2006·Surgical Infections·Jean-Louis Vincent
Apr 24, 2007·Critical Care : the Official Journal of the Critical Care Forum·Fernando A BozzaPatrícia T Bozza
Jun 30, 2010·The Journal of Cell Biology·Michael R Elliott, Kodi S Ravichandran
Feb 22, 2011·Journal of the American Society of Nephrology : JASN·Diane L Rosin, Mark D Okusa
May 13, 2011·Kidney International·Andrea Havasi, Steven C Borkan
Aug 15, 2012·Immunological Reviews·Daolin TangMichael T Lotze
Nov 9, 2012·Critical Care : the Official Journal of the Critical Care Forum·Nicolas NesselerPhilippe Seguin
Mar 22, 2013·Critical Care : the Official Journal of the Critical Care Forum·Gordon P OttoRalf A Claus
Mar 29, 2013·Critical Care : the Official Journal of the Critical Care Forum·François BelonclePierre Asfar
Aug 30, 2013·The New England Journal of Medicine·Derek C Angus, Tom van der Poll
Oct 22, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Dror MevorachReuven Or
Dec 5, 2013·Shock·Florin L CraciunDaniel G Remick
Mar 13, 2014·International Reviews of Immunology·Jun YanShulin Li
Apr 15, 2014·BMC Research Notes·Bradly ShrumTina Mele
May 20, 2014·JAMA : the Journal of the American Medical Association·Vincent LiuTheodore J Iwashyna
Jul 9, 2014·Cytokine & Growth Factor Reviews·Shih-Chin ChengMihai G Netea
Dec 19, 2014·Journal of Intensive Care·Younsuck Koh
May 1, 2015·Drug Design, Development and Therapy·Ignacio Martin-LoechesAntonio Artigas
Jul 2, 2015·Cytometry. Part B, Clinical Cytometry·Guillaume Monneret, Fabienne Venet
Sep 29, 2015·American Journal of Respiratory and Critical Care Medicine·Carolin FleischmannUNKNOWN International Forum of Acute Care Trialists
Dec 20, 2015·Immunological Reviews·Kristen K Penberthy, Kodi S Ravichandran
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Mervyn SingerDerek C Angus
Mar 8, 2016·Nature Immunology·Shih-Chin ChengMihai G Netea
Mar 10, 2016·Blood·Theodora A M ClaushuisUNKNOWN Molecular Diagnosis and Risk Stratification of Sepsis Consortium
Jun 16, 2016·Surgical Infections·Anthony J LewisMatthew R Rosengart
Jul 20, 2016·Intensive Care Medicine Experimental·Stéphanie RuizVincent Minville
Jan 1, 2014·Burns and Trauma·Dawei WangYongming Yao
Jan 25, 2017·Nature Reviews. Disease Primers·Richard S HotchkissJean-Louis Vincent
Apr 25, 2017·Nature Reviews. Immunology·Tom van der PollMihai G Netea
Apr 27, 2017·ILAR Journal·Julie A StortzPhilip A Efron
Jun 3, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fatemeh FattahiPeter A Ward
Jun 18, 2017·Cell·Sebastian WeisMiguel P Soares
Jun 29, 2017·The New England Journal of Medicine·Konrad ReinhartSimon Finfer
Aug 23, 2017·The Lancet Infectious Diseases·Flavia R MachadoUNKNOWN Latin American Sepsis Institute Network
Aug 25, 2017·Journal of Biomedical Science·Semjon SeemannAmelie Lupp
Sep 14, 2017·JAMA : the Journal of the American Medical Association·Chanu RheeUNKNOWN CDC Prevention Epicenter Program
Oct 13, 2017·Advances in Therapy·Jordi RelloSilvia Moyano
Nov 10, 2017·Frontiers in Immunology·Uriel Trahtemberg, Dror Mevorach
Nov 19, 2017·Critical Care Clinics·Jean-Louis Vincent, David Grimaldi

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Citations

Aug 13, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yang FuBinwu Ying

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Methods Mentioned

BETA
leukapheresis
flow cytometry
ELISA

Software Mentioned

GraphPad Prism
Mode

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