Apoptotic genes expression in the lumbar dorsal horn in a model neuropathic pain in rat

Neuroreport
Sabatino MaioneFrancesco Rossi

Abstract

This study combines behavioural, molecular and morphological approaches to assess the occurrence of apoptosis in the rat spinal cord by 14-day sciatic nerve chronic constrictive injury (CCI). Thermal allodynia developed in the corresponding footpad 2-3 days after surgery, while morphological features, evaluated 14 days later, consisted in a decrease (23 +/- 7%) in laminae I-III cell number ipsilateral to CCI. Apoptosis occurrence was possibly suggested by the presence of some TUNEL-positive nuclei in this territory. The mRNA expression levels of the bcl-2 genes family was changed as follows: bax increased up to 40% in CCI vs the sham rats, while bcl-2 did not change; bcl-xS massively decreased (by 70% and 100%), while bcl-xL increased (by 40%) in CCI rats. Western blot analysis showed no change either on poly-ADP ribose polymerase (PARP) or p53 transcription factor in CCI and sham rats. These data suggest that in a chronic pain condition, where the acute phase has already resolved, specific apoptotic genes are still operative and possibly may serve as a critical change for cells surviving in the chronic pain state.

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Citations

Aug 18, 2006·Psychopharmacology·Darnell T Josiah, Michelle A Vincler
Nov 20, 2003·Neuroscience and Biobehavioral Reviews·Damiana LeoCarla Perrone-Capano
Nov 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mai Lan LeongMartin Wessendorf
Jun 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christine Patte-MensahAyikoe G Mensah-Nyagan
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Apr 14, 2021·Neurochemical Research·Mahnoush KolahdouzBahareh Amin

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