Apoptotic Melanoma B16-F1 Cells Induced by Lidamycin Could Initiate the Antitumor Immune Response in BABL/c Mice.

Oncology Research
Jian-lin YangYanlin Wang

Abstract

In the process of tumor cell apoptosis induced by specific regents, calreticulin (CRT) was transferred from endoplasmic reticulum (ER) onto the cell membrane. These tumor cells, when used as the cellular vaccine to immunize experimental animals, could initiate effective antitumor immunoresponse against homologous tumor cells. This is referred to as immunogenic cell death. Lidamycin (LDM) is an enediyne antibiotic, which has extremely potent cytotoxicity to cancer cells. In this study, the mouse melanoma B16-F1 cancer cells were used to investigate the ability of LDM in promoting immunogenic cell death. Our data showed that LDM could induce apoptosis of B16-F1 cancer cells, accompanied by CRT translocation onto the cell membrane. These LDM-treated B16-F1 cells could be recognized and phagocytosed more efficiently by macrophage and dendritic cells. When the LDM-treated apoptotic B16-F1 cells were used as a whole-cell tumor vaccine to immune mice, the mice obtained resistance against rechallenged B16-F1 living cells. At the same time, the specific antitumor immune response was observed in these vaccinated mice. The splenocytes from the mice vaccinated with LDM-treated B16-F1 cells showed significantly enhanced NK lymphocyte activi...Continue Reading

References

Oct 1, 1992·Bioscience, Biotechnology, and Biochemistry·N SakataT Otani
May 6, 2004·Molecular Cell·Kirsten LauberSebastian Wesselborg
Jul 5, 2005·World Journal of Gastroenterology : WJG·Yun-Hong HuangYong-Su Zhen
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Dec 17, 2009·Cellular & Molecular Immunology·Chunyu CaoYanlin Wang
Nov 20, 2012·Annual Review of Immunology·Guido KroemerLaurence Zitvogel

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Citations

Dec 7, 2017·Frontiers in Immunology·Kristian M Hargadon

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Methods Mentioned

BETA
Assay
flow cytometry
ELISA

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