Apoptotic response of eosinophils in chronic eosinophilic pneumonia

The European Respiratory Journal
N SaitaMitsuomi Hirashima

Abstract

To clarify the pathogenesis of chronic eosinophilic pneumonia (CEP), the apoptosis of eosinophils from bronchoalveolar lavage (BAL-Eos) was compared with that of eosinophils from peripheral blood (PB-Eos) in six cases of CEP. The survival rate of eosinophils and the percentage of apoptotic cells of both types of eosinophils were examined, and the effects of interleukin 5 (IL-5) were evaluated. The role of Fas expression in apoptosis of these eosinophils was also studied. The survival rate of BAL-Eos on the third day of culture was significantly higher than that of PB-Eos (p < 0.01). This was associated with a lower proportion of apoptotic cells in BAL-Eos than in PB-Eos; the percentages of apoptotic cells in PB-Eos and BAL-Eos after 24 h of incubation were 21.7 +/- 3.4% and 10.6 +/- 1.7% respectively. IL-5 suppressed apoptosis and increased the survival rate of both PB-Eos and BAL-Eos. It was found that the apoptotic character of BAL-Eos differed from that of PB-Eos in at least three ways. Firstly, the positive rate of Fas expression on PB-Eos was increased after 24 h of incubation, whereas that on BAL-Eos did not change. Secondly, the expression of Fas on PB-Eos was suppressed by IL-5 (18.5 +/- 4.2% - 8.3 +/- 3.2%, p < 0.05), ...Continue Reading

Citations

Feb 3, 2007·Southern Medical Journal·Mahmood Alam, Nausherwan K Burki
Oct 5, 2012·Clinical Microbiology Reviews·Praveen Akuthota, Peter F Weller
Jul 21, 2009·Medizinische Klinik·Ertunc AltiokJoachim Kindler
Jun 4, 2005·Allergy·V Cottin, J-F Cordier
Dec 31, 2016·Annals of the American Thoracic Society·Inderjit Singh, Robert Kaner
Dec 25, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kentaro MachidaYoichi Nakanishi
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carrie LangdonCarl D Richards
Apr 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jiyoung Oh, James S Malter
Mar 21, 2021·Archives of Biochemistry and Biophysics·Jian-Bo ShaoPing-Chang Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Arthritis and Rheumatism
Thierry Appelboom, Joäelle Margaux
Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
© 2021 Meta ULC. All rights reserved