Apoptotic Retinal Ganglion Cell Death After Optic Nerve Transection or Crush in Mice: Delayed RGC Loss With BDNF or a Caspase 3 Inhibitor

Investigative Ophthalmology & Visual Science
M C Sánchez-MigallónMarta Agudo-Barriuso

Abstract

To investigate retinal ganglion cell (RGC) survival and activation of caspase 3 after optic nerve crush (ONC) or transection (ONT) and treatment with brain-derived neurotrophic factor (BDNF) or Z-DEVD_fmk. In albino Swiss mice, the left optic nerve was severed or crushed at 0.5 mm from the optic head and retinas were analyzed from 1 to 10 days. Additional groups were treated intravitreally with a single injection of BDNF (2.5 μg) or Z-DEVD_fmk (125 ng) right after injury, or with Z-DEVD_fmk at day 2, or with multiple injections of Z-DEVD_fmk. As controls intact or vehicle-treated retinas were used. In all retinas, Brn3a (RGCs) and cleaved-caspase 3 (c-casp3) were immunodetected and their numbers quantified. In an additional group, c-casp3 expression was assessed in RGCs retrogradely labeled before axotomy. The temporal loss of RGCs was the same after ONC or ONT and occurred in two phases with 65% loss during the first 7 days and an additional 4% loss from day 7 to 10. The appearance of c-casp3+RGCs is Gaussian, peaking at 4 days and declining thereafter. Brn3a down-regulates when RGCs start expressing c-casp3. Retinal ganglion cell rescue rate for BDNF or Z-DEVD_fmk is similar and both delay RGC loss by 1 day. Delayed treatment...Continue Reading

Citations

Mar 26, 2016·Neuroscience·Natasha Najwa Nor ArfuzirNafeeza Mohd Ismail
Mar 17, 2009·Cell Communication and Signaling : CCS·Thomas J LukasArthur H Neufeld
Apr 28, 2016·Experimental Eye Research·Ana C DordeaEmmanuel S Buys
Jul 28, 2016·Experimental Eye Research·Ushasree PattamattaAndrew White
Apr 22, 2009·Brain Research Bulletin·Thomas V Johnson, Stanislav I Tomarev
Jan 12, 2017·Expert Opinion on Drug Discovery·Renu Agarwal, Puneet Agarwal
Dec 9, 2016·Scientific Reports·Francisco M Nadal-NicolásMarta Agudo-Barriuso
Jan 13, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Najam A Sharif
Nov 11, 2017·Journal of Neuroinflammation·Francisco M Nadal-NicolásMarta Agudo-Barriuso
Dec 15, 2017·Cell Death and Differentiation·Chao LiuGavin S Dawe
Aug 5, 2017·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Carol B TorisCheryl L Rowe-Rendleman
Oct 12, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Eloísa HerreraVerónica Murcia-Belmonte
Jan 15, 2019·Investigative Ophthalmology & Visual Science·Stephanie N BlandfordSpring R M Farrell
Jun 30, 2016·Scientific Reports·Masayuki YasudaToru Nakazawa
Nov 17, 2019·International Journal of Molecular Sciences·Fernando Lucas-RuizMarta Agudo-Barriuso
Aug 27, 2017·Scientific Reports·Sheik Pran Babu Sardar PashaMike O Karl
Jul 11, 2019·Neural Regeneration Research·Fernando Lucas-RuizMarta Agudo-Barriuso
Jan 1, 2020·Translational Vision Science & Technology·Francisco J Valiente-SorianoManuel Vidal-Sanz
Jun 23, 2019·International Journal of Molecular Sciences·Beatriz Vidal-VillegasManuel Vidal-Sanz
Mar 1, 2018·Physiological Reviews·Michael FrickerGuy C Brown
May 12, 2017·Frontiers in Neuroscience·Manuel Vidal-SanzMarta Agudo-Barriuso
Nov 5, 2019·Frontiers in Neuroscience·Fernando Lucas-RuizMarta Agudo Barriuso
Jul 29, 2017·Expert Review of Ophthalmology·B A MysonaK E Bollinger
Apr 21, 2018·Cell Death Discovery·Chloe N ThomasZubair Ahmed
Sep 11, 2020·Experimental Eye Research·Leandro C Teixeira-PinheiroMarcelo F Santiago
Feb 21, 2021·Experimental Eye Research·Junhui ShenKe Yao
Apr 4, 2021·Cells·Salvatore L StellaStephanie L Grillo
Apr 4, 2019·Experimental Eye Research·Giuseppe RovereMarta Agudo-Barriuso
Apr 29, 2021·Neural Regeneration Research·Mohamed TawfikBernhard A Sabel

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