Apoptotic tumor cells are superior to tumor cell lysate, and tumor cell RNA in induction of autologous T cell response in B-CLL

Leukemia
P KokhaeiH Mellstedt

Abstract

B cell chronic lymphocytic leukemia (B-CLL) is a chronic leukemia manifested by increased numbers of B cells in circulation. The slow, smouldering nature of the disease in a significant proportion of the cases makes it an ideal target for immunotherapy. Dendritic cell (DC)-based immunotherapy is emerging as an exciting modality with significant clinical potential. In this study, three strategies for delivering antigens to DC, namely apoptotic bodies (Apo-DC), tumor lysates, and tumor RNA were studied in an autologous setting. In all six CLL patients, Apo-DC induced higher HLA-restricted, T cell responses than DC pulsed with tumor lysate or RNA. Real-time PCR confirmed higher expression of genes for IL-2 and IFN-gamma in T cells stimulated with Apo-DC. Concurrently, no IL-10 and low IL-4 responses indicated that the immune response was primarily of the Th1 type. Enzyme-linked immunospot assay revealed high IFN-gamma secretion by T cells when Apo-DC was used to stimulate autologous T cells in all patients. Our data suggest that cellular vaccines with DC loaded with apoptotic bodies may be a suitable approach for immunotherapy of B-CLL.

References

Apr 1, 1996·The Journal of Experimental Medicine·J M Austyn
Mar 1, 1996·Immunology Today·T R Mosmann, S Sad
Feb 19, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·G MeinhardtM Hallek
Sep 6, 2000·Nature Medicine·A Biragyn, L W Kwak
Dec 18, 2001·International Journal of Cancer. Journal International Du Cancer·R M Steinman, M Dhodapkar
Feb 28, 2002·Annual Review of Immunology·Pierre GuermonprezSebastian Amigorena
Apr 12, 2003·Leukemia & Lymphoma·David G SavageGwen Nichols
Apr 26, 2003·Blood·Toshiaki HayashiKenneth C Anderson
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Riikka LundRiitta Lahesmaa

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Citations

May 21, 2005·Cancer Immunology, Immunotherapy : CII·Håkan Mellstedt, Aniruddha Choudhury
Jul 30, 2008·Cancer Immunology, Immunotherapy : CII·M PalmaA Choudhury
Jul 20, 2007·Blood·Arnon P KaterThomas J Kipps
Feb 6, 2014·International Immunology·Magdalena HagnBernd Jahrsdörfer
May 13, 2014·Immunotherapy·Dige Andom Tesfatsion
Oct 31, 2006·Leukemia & Lymphoma·Krzysztof Giannopoulos, Michael Schmitt
Aug 25, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·João ConstantinoBruno M Neves
Dec 8, 2015·Immunotherapy·Sophie K HorrevortsTanja D de Gruijl
Jan 9, 2008·Seminars in Hematology·Elaine M Sloand, Katayoun Rezvani
Dec 24, 2005·Pathology·Kristen J RadfordDerek N J Hart
Nov 22, 2012·Oncoimmunology·Lorenzo GalluzziGuido Kroemer
Nov 29, 2013·Oncoimmunology·Erika VacchelliLorenzo Galluzzi
Jan 28, 2015·Human Vaccines & Immunotherapeutics·Athalia R PyzerJacalyn Rosenblatt
May 6, 2015·Oncoimmunology·Norma BloyLorenzo Galluzzi
May 26, 2016·Expert Opinion on Biological Therapy·Myrna R Nahas, David Avigan
Jan 28, 2017·Immunotherapy·Jean-Marc HoffmannAnita Schmitt
Sep 13, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·William G WierdaMichael J Keating
Mar 24, 2018·European Journal of Haematology·Marzia PalmaHåkan Mellstedt
Jun 30, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Duane A Mitchell, John H Sampson
May 28, 2020·Cancers·Benjamin MotaisJuli R Bagó
May 22, 2018·Frontiers in Immunology·Morad-Remy Muhsin-Sharafaldine, Alexander D McLellan
Aug 16, 2017·Oncoimmunology·Abhishek D GargLorenzo Galluzzi
Oct 28, 2019·Oncoimmunology·Jenny SprootenAbhishek D Garg
Apr 4, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Adam A WaltersKhuloud T Al-Jamal
Apr 16, 2021·Frontiers in Immunology·Sarah I M SutherlandGeorgina J Clark
Nov 20, 2016·Veterinary Immunology and Immunopathology·J R YannelliD A Cohen

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