Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle

Neurochemistry International
P M PaezJ M Pasquini

Abstract

In the CNS, transferrin (Tf) is expressed by the oligodendroglial cells (OLGcs) and is essential for their development. We have previously shown that apotransferrin (aTf) accelerates maturation of OLGcs in vivo as well as in vitro. The mechanisms involved in this action appear to be complex and have not been completely elucidated. The aim of this study was to investigate if Tf participates in the regulation of the cell cycle of oligodendroglial progenitor cells (OPcs). Primary cultures of OPcs were treated with aTf and/or with different combinations of mitogenic factors. Cell cycle progression was studied by BrdU incorporation, flow cytometry and by the expression of cell cycle regulatory proteins. Apotransferrin decreased the number of BrdU+ cells, increasing the cell cycle time and decreasing the number of cells in S phase. The cell cycle inhibitors p27kip1, p21cip1 and p53 were increased, and in agreement with these results, the activity of the complexes involved in G1-S progression (cyclin D/CDK4, cyclin E/CDK2), was dramatically decreased. Apotransferrin also inhibited the mitogenic effects of PDGF and PDGF/IGF on OPcs, but did not affect their proliferation rate in the presence of bFGF, bFGF/PDGF or bFGF/IGF. Our results ...Continue Reading

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Citations

Apr 6, 2012·PloS One·Lucas SilvestroffJuana María Pasquini
Jan 26, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M Guardia ClausiJ M Pasquini
Jan 18, 2015·Experimental Neurology·L N MarzialiJ M Pasquini
Dec 31, 2008·Journal of Neuroscience Research·M J PerezJ M Pasquini
Oct 7, 2008·Glia·Bozho TodorichJames R Connor
Mar 30, 2011·Glia·Bozho TodorichJames R Connor

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