PMID: 3748776Aug 1, 1986Paper

Apparent chloride conductance of subconfluent Madin Darby canine kidney cells

Pflügers Archiv : European journal of physiology
F LangM Paulmichl

Abstract

In incompletely confluent Madin Darby canine kidney (MDCK)-cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular chloride concentration. During control conditions mimicking in vivo situation, PD averages -50.3 +/- 0.7 mV. Reduction of extracellular chloride concentration from 122 mmol/l to 64.5 mmol/l depolarizes the cell membrane by +1.8 +/- 0.2 mV while reduction to 16 mmol/l leads to a transient, variable depolarization followed by a hyperpolarization of the cell membrane by -11.8 +/- 1.4 mV. 1 mmol/l anthracene-9-COOH hyperpolarizes the cell membrane by -10.7 +/- 1.0 mV, and abolishes the effect of altered extracellular chloride concentration (-0.6 +/- 0.5 mV), 1 mumol/l diphenylamine-2-carboxylate hyperpolarizes the cell membrane by -11.7 +/- 1.4 mV. 10 mumol/l furosemide hyperpolarize the cell membrane by -11.4 +/- 1.4 mV. Step increases of extracellular potassium concentration from 5.4 to 20 mmol/l depolarize the cell membrane by +14.9 +/- 1.0 mV in the absence of inhibitors, by +24.2 +/- 1.3 mV in the presence of anthracene-9-COOH and by +28.8 +/- 0.7 mV in the presence of furosemide. 10 mumol/l isoprotere...Continue Reading

References

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Citations

Aug 1, 1992·Pflügers Archiv : European journal of physiology·N L Simmons, D R Tivey
Dec 1, 1991·Pflügers Archiv : European journal of physiology·N L Simmons
Aug 1, 1991·Pflügers Archiv : European journal of physiology·L Wojnowski, H Oberleithner
Jul 1, 1990·Pflügers Archiv : European journal of physiology·H OberleithnerU Kersting
Apr 1, 1987·Pflügers Archiv : European journal of physiology·M PaulmichlF Lang
Apr 1, 1988·Pflügers Archiv : European journal of physiology·M PaulmichlF Lang
Mar 1, 1989·Pflügers Archiv : European journal of physiology·M PaulmichlF Lang
Dec 1, 1988·The Journal of Membrane Biology·F FriedrichF Lang
Oct 11, 1988·Biochimica Et Biophysica Acta·H Gögelein
Feb 1, 1995·Cellular Signalling·F BelachgarT Anagnostopoulos
May 1, 1996·British Journal of Pharmacology·M GschwentnerM Paulmichl
Oct 1, 1995·Kidney International·F Lang, M Paulmichl
Aug 1, 1992·Kidney International·W L MaciasW M Armstrong
Sep 13, 2003·The Journal of Biological Chemistry·Markus RitterMarkus Paulmichl
Mar 19, 1992·Nature·M PaulmichlD Clapham
May 1, 1991·The American Journal of Physiology·M SteidlF Lang
Jul 1, 1990·Pflügers Archiv : European journal of physiology·H OberleithnerW Steigner
Jul 1, 1990·Pflügers Archiv : European journal of physiology·H OberleithnerW Pfaller
Oct 28, 1998·Physiological Reviews·L P SullivanJ J Grantham
May 1, 1989·The American Journal of Physiology·F FriedrichF Lang
Oct 1, 1991·The American Journal of Physiology·M RitterF Lang
Nov 4, 1991·Biochimica Et Biophysica Acta·H WeissF Lang

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