Apr 11, 1984

Apparent saturation kinetics of divalent cation uptake in yeast caused by a reduction in the surface potential

Biochimica Et Biophysica Acta
G W Borst-Pauwels, A P Theuvenet

Abstract

The concentration dependence of the uptake rate of divalent cations in yeast can be described by a simple diffusion process after accounting for the effect of the surface potential upon the divalent cation concentration near the membrane. It is also necessary to correct for the effect of the cell pH upon the rate of translocation. The apparent saturation kinetics is ascribed to the fact that the quotient of the concentration of the divalent cations near the cell membrane and the bulk aqueous phase concentration is reduced on increasing the divalent cation concentration in the medium. The diffusion process regulated by the surface potential even mimics the saturation kinetics of a two-carrier transport system. The selectivity found between Ca2+ and Sr2+ uptake can probably be traced to differences in their affinity for the negative groups on the cell membrane determining the surface potential rather than to differences in their affinity for a transport system. The enhancement of divalent cation uptake by loading the cells with phosphate is probably due to the concomitant increase in the net negative charge of the cell membrane.

  • References7
  • Citations7

Mentioned in this Paper

Cations, Divalent
Calcium
Resting Potentials
Uptake
Phosphate Measurement
Etiology
Mimic brand of tebufenozide
Intracellular Translocation
Saccharomyces cerevisiae
Phosphate ion

About this Paper

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.