Apparent size and morphology of bacterial microcompartments varies with technique

PloS One
Nolan W. KennedyDanielle Tullman-Ercek

Abstract

Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies.

References

Feb 1, 1970·Journal of Bacteriology·J M ShivelyJ W Greenawalt
Sep 1, 1994·Journal of Bacteriology·P ChenJ R Roth
Aug 6, 2005·Science·Cheryl A KerfeldTodd O Yeates
Oct 10, 2006·Journal of Molecular Biology·Michael F SchmidJessup M Shively
Feb 23, 2008·Science·Shiho TanakaTodd O Yeates
Mar 12, 2008·The Journal of Biological Chemistry·Joshua B ParsonsMichael B Prentice
Oct 22, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shouqiang ChengThomas A Bobik
Aug 4, 2009·Nature Biotechnology·John E DueberJay D Keasling
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Walter BonacciDavid F Savage
Mar 20, 2012·PloS One·Swati ChoudharyClaudia Schmidt-Dannert
Aug 11, 2012·Journal of the Royal Statistical Society. Series A, (Statistics in Society)·M J Sweeting, S G Thompson
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 29, 2012·Protein Science : a Publication of the Protein Society·Julien JordaTodd O Yeates
Aug 8, 2013·Journal of Molecular Microbiology and Biotechnology·Mark HeldClaudia Schmidt-Dannert
Mar 19, 2014·Journal of Molecular Biology·Jonathan K LassilaCheryl A Kerfeld
May 21, 2014·ELife·Niall Mangan, Michael Brenner
May 31, 2014·The Journal of Biological Chemistry·Allan PangRichard W Pickersgill
Jul 24, 2014·Protein Science : a Publication of the Protein Society·Edward Y KimDanielle Tullman-Ercek
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Chiranjit ChowdhuryThomas A Bobik
Oct 24, 2014·PLoS Computational Biology·Seth D AxenCheryl A Kerfeld
Jan 2, 2015·Circulation Research·C Glenn Begley, John P A Ioannidis
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chiranjit ChowdhuryThomas A Bobik
Feb 6, 2016·Nature·David B AllisonKathryn A Kaiser
Jun 3, 2016·Science Translational Medicine·Steven N GoodmanJohn P A Ioannidis
Jul 28, 2016·Applied Microbiology and Biotechnology·Maureen B QuinClaudia Schmidt-Dannert
Jun 7, 2017·ACS Synthetic Biology·Marilyn F Slininger LeeDanielle Tullman-Ercek

❮ Previous
Next ❯

Citations

Sep 13, 2020·Biotechnology and Bioengineering·Jesse A Jones, Tobias W Giessen
Nov 24, 2020·Journal of Molecular Biology·Nolan W KennedyDanielle Tullman-Ercek
Jan 16, 2021·Trends in Biochemical Sciences·Andrew M StewartThomas A Bobik
Sep 5, 2020·Protein Science : a Publication of the Protein Society·Jessica M OchoaTodd O Yeates
Mar 9, 2021·ACS Nano·Farzaneh MohajeraniMichael F Hagan
Mar 26, 2021·Protein Science : a Publication of the Protein Society·Eva Emilija CesleGints Kalnins
Apr 5, 2021·Current Opinion in Microbiology·Hao ChenChenguang Fan
Oct 9, 2020·EcoSal Plus·Katie L StewartThomas A Bobik
Jun 1, 2021·Current Opinion in Microbiology·Jessica M Ochoa, Todd O Yeates
Jun 1, 2021·ACS Central Science·Yaohua LiMonica Olvera de la Cruz
Jun 25, 2021·Current Opinion in Microbiology·Gaurav Kumar, Sharmistha Sinha
Jun 15, 2021·Current Opinion in Microbiology·Nolan W KennedyDanielle Tullman-Ercek

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
transmission
scanning electron microscopy
dynamic light scattering
transmission electron microscopy
imaging techniques
light scattering

Software Mentioned

Excel
Zeta Sizer
ImageJ
R
Nanosight

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Related Papers

BioRxiv : the Preprint Server for Biology
Nolan W. KennedyDanielle Tullman-Ercek
Protein Science : a Publication of the Protein Society
Edward Y KimDanielle Tullman-Ercek
Proceedings of the National Academy of Sciences of the United States of America
Chenguang FanThomas A Bobik
© 2021 Meta ULC. All rights reserved