PMID: 9165301Jan 1, 1997Paper

Appearance of 1-2 Mbp giant DNA fragments as an early common response leading to cell death induced by various substances that cause oxidative stress

Free Radical Biology & Medicine
Y Higuchi, S Matsukawa

Abstract

The effects of oxidative stress on double strand DNA breakage were examined in T-24 human bladder tumor cells using various active oxygen producing agents such as hydrogen peroxide (H2O2), bleomycin (BLM), neocarzinostatin (NCS), and x-ray irradiation. Analysis of the DNA by pulsed-field gel electrophoresis (PFGE) revealed that discrete giant DNA fragments of 1-2 Mbp and 200-800 kbp had accumulated in the nuclei of the treated cells. The 1-2 Mbp giant DNA fragments were first observed 2 h after the T-24 cells were exposed to the active oxygen producing agents, or irradiated with x-ray. The appearance and the amounts of 1-2 Mbp and 200-800 kbp giant DNA fragments seemed to depend on the concentration and the type of reagents used or the dose of x-ray. Following the accumulation of giant DNA fragments, another type of DNA fragmentation was detected and DNA fragments smaller than 100 kbp accumulated in the nuclei of the cells irradiated with x-ray or treated with NCS. In addition, DNA ladder formation, which is characteristic of apoptosis, was observed. The giant DNA fragments appeared to arise as a consequence of double-stranded DNA breakage, which occurred earlier than cell lysis, as assessed by 51Cr release. These findings indi...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A D D'Andrea, W A Haseltine
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J Steighner, L F Povirk
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L M SchwartzB K Kay
Apr 27, 1989·Nature·W R Brown
Jan 1, 1985·Annual Review of Biochemistry·J C Wang
Nov 1, 1988·Trends in Biochemical Sciences·V A Bohr, K Wassermann
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J H Doroshow
Jul 1, 1984·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P E Bryant
Feb 24, 1984·Biochimica Et Biophysica Acta·A C Mello Filho, R Meneghini
Feb 15, 1984·The Biochemical Journal·A C Mello FilhoR Meneghini
Dec 31, 1982·Biochimica Et Biophysica Acta·L V NikonovaS R Umansky
Nov 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S PandeyM Sikorska
Jul 15, 1994·Biochemical and Biophysical Research Communications·B ZhivotovskyS Orrenius
Jan 1, 1993·Archives of Biochemistry and Biophysics·M A Barry, A Eastman
Apr 1, 1986·Immunology Today·E Duvall, A H Wyllie

❮ Previous
Next ❯

Citations

Oct 14, 2003·Biochemical Pharmacology·Yoshihiro Higuchi
Feb 13, 2001·Brain Research·D MaulikM Delivoria-Papadopoulos
Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G Mathé
Dec 21, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Joachim VolkWerner Geurtsen
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L FalascaL Conti Devirgiliis
Jan 29, 2003·Environmental and Molecular Mutagenesis·Sophie MeintièresDaniel Marzin
Sep 28, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tsan-Zon LiuChi-Liang Chern
Feb 10, 2007·Seminars in Nuclear Medicine·Drew A Torigian, Abass Alavi
Dec 17, 2004·Journal of Cellular and Molecular Medicine·Yoshihiro Higuchi
Apr 16, 2009·Journal of Biophotonics·Rajaram Krishnan, Michael J Heller
Mar 11, 2006·Mechanisms of Ageing and Development·Ian Martin, Michael S Grotewiel
Nov 12, 2013·Water Research·Rajni Kaushik, Rajasekhar Balasubramanian
May 23, 1998·Experimental Cell Research·D L ChenK H Sit
Mar 6, 1999·The Journal of Biological Chemistry·W FuM P Mattson
Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H SawadaS Shimohama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis