Appearance of a novel Ca2+ influx pathway in Sf9 insect cells following expression of the transient receptor potential-like (trpl) protein of Drosophila

Biochemical and Biophysical Research Communications
Y HuW P Schilling

Abstract

Activation of phospholipase C, elevation of free cytosolic Ca2+ concentration ([Ca2+]i) and stimulation of Ca2+ influx have been implicated in Drosophila phototransduction. Electrophysiological studies suggest that trp and trpl proteins may be important for the light-activated Ca2+ current found in Drosophila photoreceptor cells. Although these proteins exhibit homologies to voltage-gated Ca2+ and Na+ channels, their actual function in insect cells and their relation to proteins involved in mammalian cell Ca2+ signaling remains unknown. In the present study, [Ca2+]i was examined in fura-2-loaded Sf9 insect cells infected with recombinant baculovirus containing cDNA for the trpl protein. Ca2+ influx was examined by use of Ba2+, a Ca2+ surrogate that is not a substrate for Ca(2+)-pumps or carriers and by measurement of whole-cell membrane currents. The results suggest that expression of trpl is associated with appearance of a Ca2+ permeable, non-selective cation channel formed by the trpl protein.

Citations

Apr 1, 1996·Molecular Neurobiology·B Minke, Z Selinger
Jan 1, 1997·The American Journal of Physiology·D L KunzeW P Schilling
Sep 25, 1999·Japanese Journal of Pharmacology·S MiwaT Masaki
Oct 13, 2000·American Journal of Physiology. Cell Physiology·A KurumaH C Hartzell
Feb 8, 2006·Annual Review of Physiology·Baruch Minke, Moshe Parnas
Jun 17, 1999·FEBS Letters·C TrostV Flockerzi
Aug 22, 2001·The Journal of Biological Chemistry·M GoelW P Schilling
Dec 28, 1999·Annual Review of Cell and Developmental Biology·C Montell
Jun 18, 2005·The Journal of Physiology·Craig Montell
Nov 14, 1997·Molecular Pharmacology·C Montell
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·B GilloB Minke
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·C Montell
Sep 10, 2013·Nature Neuroscience·Yali V ZhangCraig Montell
Feb 3, 2011·Pflügers Archiv : European journal of physiology·Roger C Hardie
Sep 3, 2020·Cells·Xingjuan ChenAlexander G Obukhov
Feb 9, 1996·The Journal of Biological Chemistry·W G SinkinsW P Schilling
Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Putney, R R McKay
Sep 23, 2006·The Journal of Physiology·Roger C Hardie
Jul 1, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Soichi MiwaTomoo Masaki
Mar 28, 2002·Physiological Reviews·Baruch Minke, Boaz Cook
Feb 1, 2020·Pharmacology & Therapeutics·Hongbo WangMichael X Zhu
Nov 1, 1994·The American Journal of Physiology·L VacaW P Schilling
Aug 11, 2012·Life Sciences·Melissa A Fowler, Craig Montell
Jun 2, 2000·Molecular and Cellular Neurosciences·P RaghuR C Hardie
Aug 1, 1996·Current Opinion in Neurobiology·B Minke, Z Selinger
Aug 4, 1998·Current Opinion in Neurobiology·C Montell
May 27, 2003·Cell Calcium·Christian Harteneck
May 27, 2003·Cell Calcium·Craig Montell

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