Abstract
Blast cells derived from blood and marrow samples of a patient with acute lymphoblastic leukemia (ALL), as well as from the Reh line originally established from an ALL patient, were cultured in diffusion chambers implanted i.p. into preirradiated CBA mice. At different intervals over a period of up to 20 days, surface immunoglobulins, ALL antigen, and T-cell antigen were investigated by using direct immunofluorescence. Rosette formation was tested with sheep and mouse erythrocytes. On day 0, the cells expressed only ALL antigen at the surface, and no rosette formation was observed. During culture the patient's lymphoblasts, which originally had cytoplasmic IgM in addition to ALL antigen, expressed surface immunoglobulins as well as mouse erythrocyte receptors. The Reh line cells were ambivalent in two experiments developing T-cell antigens and sheep erythrocyte receptors as well as mouse erythrocyte receptors. Our data suggest that the differentiation arrest in leukemic lymphoblasts can be overcome, thus entailing a surface pattern similar to mature T- or B-lymphocytes.
References
Feb 4, 1978·Lancet·W R BoeckerC G Schmidt
Sep 10, 1977·Lancet·A V HoffbrandR K Woodruff
Jun 30, 1977·Nature·C RosenfeldM F Greaves
Feb 19, 1979·Blut·G JägerP Dörmer
Jan 1, 1977·Haematology and Blood Transfusion·H RodtS Thierfelder
Apr 20, 1978·The New England Journal of Medicine·L B VoglerM D Cooper
Apr 1, 1978·Clinical Immunology and Immunopathology·J Kaplan, W D Peterson
Jun 20, 1978·Blut·E ThielS Thierfelder
Apr 10, 1975·The New England Journal of Medicine·R McCaffreyD Baltimore
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S GuptaF P Siegal
Aug 28, 1975·The New England Journal of Medicine·I Tsukimote, B C Lampkin
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·U HämmerlingJ Abbott
May 1, 1975·Clinical Immunology and Immunopathology·M F GreavesT A Lister
Aug 1, 1972·The Journal of Experimental Medicine·M JondalH Wigzell
Apr 6, 1974·Lancet·G Stathopoulos, E V Elliott
Jan 1, 1973·Transplantation Reviews·M SeligmannJ C Brouet
Oct 1, 1973·The Journal of Experimental Medicine·M P ScheidA L Goldstein
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J F BachA White
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Dec 28, 1973·Science·J H KerseyM E Nesbit
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M ParanP Resnitzky
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato