PMID: 8603935Nov 1, 1995Paper

Appearance of interleukin 1 alpha relates DNA interstrand cross-links and cytotoxicity in cultured human keratinocytes exposed to bis-(2-chloroethyl) sulfide

Journal of Applied Toxicology : JAT
Y PuI A Bernstein

Abstract

The utility of an increase in the level of interleukin 1 alpha (IL-1 alpha) as an indicator of cytotoxicity from exposure to bis-(2-chloroethyl) sulfide (BCES) was evaluated in submerged monolayer cultures of human cutaneous keratinocytes. Four-day-old cultures were exposed to 1-100 microM BCES at 37 degrees for 30 min. The amounts of IL-1 alpha in the medium at and in cells 72 h after exposure were measured immunologically with an enzyme-linked immunosorbent assay using monoclonal antibody to human IL-1 alpha. The antibody was conjugated with peroxidase for visualization. Cell viability was measured concomitantly using the trypan blue exclusion technique. The degree of interstrand cross-linking as a measure of damage in the cellular DNA was determined by measuring the fluorescence resulting from the intercalation of ethidium bromide into double-stranded molecules that remained in heat-denatured DNA isolated from cells that had been exposed to BCES. A high correlation was observed between the dose-responsive increase in the level of IL-1 alpha in the medium and in the cells, and the dose-responsive decrease that took place in the fraction of viable cells in exposed cultures. The dose-responsive increase in the interstrand cross...Continue Reading

References

Jan 26, 1991·Lancet·J N BarkerB J Nickoloff
Jan 1, 1987·The Journal of Investigative Dermatology·D GoldminzJ McGuire
Apr 1, 1984·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R F VogtB Papirmeister
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L C EricksonK W Kohn
Mar 1, 1980·Mutation Research·M Fox, D Scott
Jul 1, 1956·Journal of Bacteriology·S ZAMENHOFH E ALEXANDER

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Citations

Nov 18, 2000·Journal of Biochemical and Molecular Toxicology·C L SabourinR P Casillas
Feb 14, 2002·Biochemical Pharmacology·Avikam HarelKonstantin Bloch
Nov 5, 2013·Seminars in Immunology·Peleg RiderRon N Apte
Sep 29, 2018·The Journal of Biological Chemistry·Jun WangWeidong Yong
Feb 19, 2011·Critical Reviews in Toxicology·Kamyar GhabiliMohammadali M Shoja

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