Apple fruit acidity is genetically diversified by natural variations in three hierarchical epistatic genes: MdSAUR37, MdPP2CH and MdALMTII

The Plant Journal : for Cell and Molecular Biology
Dongjie JiaZhenhai Han

Abstract

Many efforts have been made to map quantitative trait loci (QTLs) to facilitate practical marker-assisted selection (MAS) in plants. In the present study, using MapQTL and BSA-seq (bulk segregant analysis using next generation sequencing) with two independent pedigree-based populations, we identified four major genome-wide QTLs responsible for apple fruit acidity. Candidate genes were screened in major QTL regions, and three functional gene markers, including a non-synonymous A/G single-nucleotide polymorphism (SNP) in the coding region of MdPP2CH, a 36-bp insertion in the promoter of MdSAUR37 and a previously reported SNP in MdALMTII, were validated to influence the malate content of apple fruits. In addition, MdPP2CH inactivated three vacuolar H+ -ATPases (MdVHA-A3, MdVHA-B2 and MdVHA-D2) and one aluminium-activated malate transporter (MdALMTII) via dephosphorylation and negatively influenced fruit malate accumulation. The dephosphotase activity of MdPP2CH was suppressed by MdSAUR37, which implied a higher hierarchy of genetic interaction. Therefore, the MdSAUR37/MdPP2CH/MdALMTII chain cascaded hierarchical epistatic genetic effects to precisely determine apple fruit malate content. An A/G SNP (-1010) on the MdMYB44 promoter ...Continue Reading

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Citations

Jan 15, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Éric DuchêneDidier Merdinoglu
Jul 11, 2018·Plant, Cell & Environment·Yi FengZhenhai Han
Apr 10, 2019·Horticulture Research·Cameron P PeaceStijn Vanderzande
Oct 5, 2020·Journal of Applied Microbiology·Xiaofen ChaiYi Wang
Nov 1, 2020·Plant Molecular Biology·Lu WangYuepeng Han
May 23, 2021·Molecular Plant·Liao LiaoYuepeng Han
Oct 7, 2021·Horticulture Research·Qingyuan DangDongjie Jia

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