Applicability and robustness of the hen's egg test for analysis of micronucleus induction (HET-MN): results from an inter-laboratory trial

Mutation Research
Daniela GreyweKerstin Reisinger

Abstract

The hen's egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG & Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the "irrelevant" positives, isophorone and 2,4-dichlorophenol ("irrelevant" means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the prom...Continue Reading

References

Jan 1, 1990·Environmental and Molecular Mutagenesis·B E AndersonK S Loveday
Jan 1, 1990·Environmental and Molecular Mutagenesis·B MyhrW J Caspary
Jan 1, 1988·Environmental and Molecular Mutagenesis·D B McGregorW J Caspary
Jan 1, 1986·Environmental Mutagenesis·K MortelmansE Zeiger
Oct 25, 1973·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G A Bruns, V M Ingram
Jun 1, 1993·Mutation Research·R BaraleI Barrai
Sep 1, 1993·Mutation Research·J D TuckerA V Carrano
May 20, 1998·Mutation Research·M J AardemaR D Taalman
Jan 6, 2000·Archives of Biochemistry and Biophysics·J C OurlinU A Meyer
Dec 13, 2000·Mutation Research·M Fenech
Dec 13, 2000·Mutation Research·G Krishna, M Hayashi
Mar 5, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Th WolfN-P Luepke
Feb 14, 2007·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Serap CeliklerNilüfer Aydemir
Oct 1, 2003·Nonlinearity in Biology, Toxicology, Medicine·Edward J Calabrese, Linda A Baldwin

❮ Previous
Next ❯

Citations

Aug 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Raffaella Corvi, Federica Madia
Jul 10, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B Çelik, H Özparlak
Mar 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A M ApiS Tsang
Oct 15, 2020·Journal of Cosmetic Dermatology·Xiao MaBo Wang
Jun 4, 2021·Mutagenesis·Kerstin ReisingerRalph Pirow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.