Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India

Veterinary Sciences
K Nithin PrabhuSira Abdul Rahman

Abstract

Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as the more sensitive, reverse transcription polymerase chain reaction (RT-PCR), have recently been employed for the laboratory diagnosis of rabies. We compared the three methods on brain samples from domestic (dog, cat, cattle, buffalo, horse, pig and goat) and wild (leopard, wolf and jackal) animals from various parts of India. Of the 257 samples tested, 167 were positive by all the three tests; in addition, 35 of the 36 decomposed samples were positive by RT-PCR. This is the first study in which such large number of animal samples have been subjected to the three tests simultaneously. The results confirm 100% corroboration between DFA and dRIT, buttress the applicability of dRIT in the simple and rapid diagnosis of rabies in animals, and reaffirm the suitability of RT-PCR for samples unfit for testing either by DFA or dRIT.

References

Apr 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A N Hamir, G Moser
Aug 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·S L Morton, D R Tindall
Nov 14, 1997·Journal of Clinical Microbiology·P R HeatonJ E Whitby
Mar 21, 1998·Journal of Virological Methods·J E WhitbyP Johnstone
May 23, 1998·Journal of Clinical Microbiology·P CrepinH Bourhy
May 30, 2002·Veterinary Microbiology·D DavidY Stram
Nov 27, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zerai Woldehiwet
Feb 24, 2006·Emerging Infectious Diseases·Tiziana LemboCharles E Rupprecht
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Edith Rojas AnayaEliseo Hernández Baumgarten
May 23, 2006·Emerging Infectious Diseases·Rafael OliveiraIvanete Kotait
Feb 13, 2007·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Carla Isabel MacedoIvanete Kotait
Jul 14, 2007·Journal of Virological Methods·BoKyu KangDaeSub Song
Sep 20, 2007·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Kimberly Moore DalalDaniel G Coit
Feb 27, 2008·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Rozario Menezes
Mar 28, 2008·PLoS Neglected Tropical Diseases·Salome DürrJakob Zinsstag
Apr 23, 2008·Microbiology and Immunology·Akira NishizonoKinjiro Morimoto
Dec 3, 2008·Emerging Infectious Diseases·Nicholas JohnsonKenneth McColl
Sep 30, 2009·PLoS Neglected Tropical Diseases·Anthony R FooksThomas Müller
Nov 11, 2009·Journal of Wildlife Diseases·Alexander SingerGraham C Smith
Mar 11, 2010·Expert Review of Molecular Diagnostics·Supaporn Wacharapluesadee, Thiravat Hemachudha
Jun 1, 2009·The Onderstepoort Journal of Veterinary Research·W MarkotterL H Nel
Jan 17, 2012·Biologicals : Journal of the International Association of Biological Standardization·Alexandre ServatFlorence Cliquet
Mar 23, 2012·Journal of Veterinary Science·Dong-Kun YangJae-Young Song
Apr 12, 2012·The American Journal of Tropical Medicine and Hygiene·Kamruddin AhmedAkira Nishizono
Oct 12, 2012·Virologica Sinica·Shampur Narayan MadhusudanaYajaman Belludi Ashwin
Apr 13, 2013·Journal of Travel Medicine·Henry WildeThiravat Hemachudha
Dec 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·R P Aravindh BabuN D J Chandran
Jul 11, 2014·Journal of Virological Methods·Lorraine M McElhinneyAnthony R Fooks
Aug 15, 2014·Journal of Infection in Developing Countries·Fernando J BeltranDaniel M Cisterna
Mar 24, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Veasna DuongPhilippe Buchy
Apr 16, 2016·Journal of Virological Methods·Jodie A JarvisApril D Davis
Sep 24, 2016·The Veterinary Record·Karen L MansfieldAbdul Rahman
Oct 6, 2016·PLoS Neglected Tropical Diseases·Monique LéchenneLaurent Dacheux

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Methods Mentioned

BETA
dot-blot
light microscopy
PCR
electrophoresis

Software Mentioned

dRIT

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