Application of 16O/18O reverse proteolytic labeling to determine the effect of biofilm culture on the cell envelope proteome of Porphyromonas gingivalis W50

Proteomics
Ching-Seng AngEric C Reynolds

Abstract

Porphyromonas gingivalis is an oral pathogen linked to chronic periodontitis. The bacterium exists as part of a polymicrobial biofilm accreted onto the tooth surface. An understanding of the changes to the proteome especially of the cell envelope of biofilm cells compared with planktonic cells could provide valuable insight into the molecular processes of biofilm formation. To establish which proteins changed in abundance between the planktonic and biofilm growth states, the cell envelope fractions of two biological replicates of P. gingivalis cultivated in a chemostat were analysed. Proteins were separated by 1-D SDS-PAGE, in-gel digested with trypsin in the presence of H216O or H218O and identified and quantified by LC-MALDI TOF/TOF-MS. Using a reverse labeling strategy we identified and quantified the changes in abundance of 81 P. gingivalis cell envelope proteins. No form of bias between the labels was observed. Twenty four proteins increased in abundance and 18 decreased in abundance in the biofilm state. A group of cell-surface located C-Terminal Domain family proteins including RgpA, HagA, CPG70 and PG99 increased in abundance in the biofilm cells. Other proteins that exhibited significant changes in abundance included t...Continue Reading

References

Jul 1, 1991·Rapid Communications in Mass Spectrometry : RCM·T TakaoY Shimonishi
May 1, 1989·Journal of Periodontal Research·M E NeidersR J Genco
May 1, 1986·Infection and Immunity·A S McKeeP D Marsh
Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Oct 17, 1998·Journal of Clinical Microbiology·A L GriffenE J Leys
Apr 17, 1999·Science·C Potera
Oct 29, 2000·Journal of Bacteriology·S G DashperE C Reynolds
Jun 5, 2001·Nature Reviews. Genetics·J Quackenbush
Jun 22, 2001·Journal of Bacteriology·S G DashperE C Reynolds
Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·I I StewartD Figeys
Jan 25, 2002·Journal of Bacteriology·Karin SauerDavid G Davies
May 7, 2003·Current Opinion in Microbiology·Kristian KvintThomas Nyström
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Dec 20, 2003·Current Protein & Peptide Science·N M O-Brien-SimpsonE C Reynolds
Apr 20, 2004·Rapid Communications in Mass Spectrometry : RCM·Marcus BantscheffBernhard Kuster
Mar 4, 2005·Proteomics·Jun SakaiMasaharu Kanaoka
Mar 9, 2005·Molecular & Cellular Proteomics : MCP·Wei-Jun QianUNKNOWN Inflammation and the Host Response to Injury Large Scale Collaborative Research Program
Apr 13, 2005·Journal of Proteome Research·Josip BlonderTimothy D Veenstra
May 20, 2005·The Journal of Biological Chemistry·Stuart G DashperEric C Reynolds
Jun 28, 2005·Journal of the American Society for Mass Spectrometry·Brian L HoodAly Karsan
Jul 16, 2005·Rapid Communications in Mass Spectrometry : RCM·Sandra KörbelEberhard Krause
Oct 7, 2005·Microbiology·Paul A Hoskisson, Glyn Hobbs
Nov 10, 2005·Journal of Periodontology·Howard K KuramitsuAki Ikegami
Dec 13, 2005·Journal of Proteome Research·Catherine RathsamNicholas A Jacques
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann
Mar 15, 2006·Proteomics·Daniel López-FerrerJesús Vázquez

❮ Previous
Next ❯

Citations

Sep 14, 2010·Journal of Industrial Microbiology & Biotechnology·Alan T Bull
Jul 20, 2012·Journal of Proteome Research·Zamirah Zainal-AbidinEric C Reynolds
Jan 12, 2010·Journal of Bacteriology·Alvin LoEric Reynolds
Oct 14, 2010·Journal of Dental Research·S G DashperE C Reynolds
May 21, 2009·BMC Microbiology·Masae KuboniwaRichard J Lamont
May 9, 2009·PLoS Pathogens·Halina WójtowiczF Xavier Gomis-Rüth
Feb 9, 2010·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Sonia B BhardwajK Gauba
Jul 20, 2012·Expert Review of Proteomics·Masae KuboniwaMurray Hackett
Jun 13, 2015·Medical Microbiology and Immunology·Arbia KhemiriPascal Cosette
Mar 23, 2010·Journal of Chromatography. a·Ondrej Pes, Jan Preisler
Jun 9, 2009·Biochimica Et Biophysica Acta·Paul D VeithEric C Reynolds
Apr 7, 2009·Biochemical and Biophysical Research Communications·Halina WójtowiczTeresa Olczak
May 5, 2009·Rapid Communications in Mass Spectrometry : RCM·Fang FangYinlong Guo
Jan 17, 2012·Proteomics·C Jayampath SeneviratneLakshman P Samaranayake
Dec 21, 2012·Molecular Oral Microbiology·C J WrightH F Jenkinson
Jul 24, 2014·Molecular Oral Microbiology·K J CrossE C Reynolds
Dec 19, 2009·Periodontology 2000·Masae Kuboniwa, Richard J Lamont
May 1, 2015·Anaerobe·Maribasappa KarchedSirkka Asikainen
Dec 21, 2016·Proteomics·Nagihan Bostanci, Kai Bao
Nov 24, 2020·Periodontology 2000·Nagihan BostanciGeorgios N Belibasakis
Apr 3, 2009·Journal of Proteome Research·Catherine Fenselau, Xudong Yao
Aug 12, 2009·Journal of Proteome Research·Paul D VeithEric C Reynolds
Oct 14, 2010·Analytical Chemistry·Ning LiuZongwei Cai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.