Application of a bacterial whole cell biosensor for the rapid detection of cytotoxicity in heavy metal contaminated seawater

Chemosphere
Zhisong CuiWei E Huang

Abstract

A toxicity biosensor Acinetobacter baylyi Tox2 was constructed with the host strain A. baylyi ADP1 harboring a new and medium-copy-number plasmid pWH1274_lux, and was applied to detect the cytotoxicity of heavy metal contaminated seawater. The gene cassette luxCDABE was controlled by constitutively expressed promoter Ptet on pWH1274_lux and the bioluminescence intensity of the biosensor reduces in proportional to the concentrations of toxic compounds. A. baylyi Tox2 exhibits tolerance to salinity, hence it is applicable to seawater samples. A. baylyi Tox2 and Mugilogobius chulae were exposed to different concentrations of heavy metals (Hg2+, Zn2+, Cu2+, and Cd2+) in artificial seawater for performance comparison and Pearson correlation analysis showed a significant correlation (p < 0.01) between A. baylyi Tox2 toxicity detection and the fish (M. chulae) exposure test. This suggests that the performance of A. baylyi Tox2 is comparable to the conventional fish toxicity test in terms of cytotoxicity detection of heavy metal contaminated seawater. Furthermore, A. baylyi Tox2 was used to evaluate cytotoxicity of field-collected seawater samples. The results indicate that there was a significant correlation between the luminescence i...Continue Reading

Citations

Jun 18, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nadine Lobsiger, Wendelin J Stark
Dec 13, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Allison L ThebergeJustin C Biffinger
Sep 23, 2020·Analytical and Bioanalytical Chemistry·Ankur Singh, Vipin Kumar
May 10, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Yuan YangYang-Chun Yong
Jan 20, 2021·Environmental Science and Pollution Research International·Qurban AliShafaqat Ali
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Yan GuoHong-Ju Zheng
Nov 17, 2021·Applied Biochemistry and Biotechnology·Shrute Kannappan, Bhaskar Chandra Mohan Ramisetty

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