Application of a competitive ELISA for the detection of bluetongue virus antibodies in llamas and wild ruminants

Journal of Wildlife Diseases
A AfsharD J Myers

Abstract

A competitive enzyme-linked immunosorbent assay (C-ELISA), using a group-specific monoclonal antibody against bluetongue virus (BTV), was applied to detect anti-BTV antibodies in serum samples from two llamas (Llama glama) experimentally infected with BTV serotype 10. Antibodies were detected in both llamas by 1 wk or 2 wk post-infection. Antibodies to BTV increased exponentially during the first 4 wk in both llamas and stabilized at an elevated level during the remaining 5-wk-period of the experiment. We evaluated the C-ELISA for 1,442 field sera from bluetongue-free areas, collected from 398 llamas in New Zealand as well as 451 elk (Cervus elaphus canadensis), 323 bison (Bison bison) and 270 reindeer (Rangifer tarandus tarandus) in Canada. Based on the frequency distribution of the C-ELISA values, we propose that the current negative cut-off value of 50% inhibition established for bovine field sera also can be applied to the sera from these wild ruminants. The C-ELISA values for other wild ruminant field sera collected in bluetongue-free areas of Canada from 98 native caribou (Rangifer tarandus caribou), 32 white-tailed deer (Odocoileus virginianus), 14 moose (Alces alces), and nine musk-oxen (Ovibos maschatus) and 15 yak (Bo...Continue Reading

Citations

Aug 1, 1994·Comparative Immunology, Microbiology and Infectious Diseases·A Afshar
Feb 1, 1997·Preventive Veterinary Medicine·S AkhtarO Thieme
Aug 5, 1998·Preventive Veterinary Medicine·R S SingerA S Fisher
Jan 14, 2003·Veterinary Microbiology·M LundervoldC Hamblin
May 30, 2008·Emerging Infectious Diseases·Francisco Ruiz-FonsChristian Gortázar
Aug 25, 2011·Veterinary Microbiology·Claudia SchulzBernd Hoffmann
Mar 18, 2011·Veterinary Microbiology·Caterina FalconiChristian Gortázar
May 23, 2009·The Veterinary Clinics of North America. Food Animal Practice·Sanjay KapilJames F Evermann
Jul 15, 2015·Polish Journal of Veterinary Sciences·W Niedbalski
Jan 28, 2014·Virus Research·Peter CoetzeeMaria Stokstad
Apr 16, 2014·Journal of Virological Methods·Marine WasniewskiFlorence Cliquet

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