PMID: 7544985Jul 1, 1995Paper

Application of a fluorescent redox dye for enumeration of metabolically active bacteria on albumin-coated titanium surfaces

Letters in Applied Microbiology
S J McDowellR J Friedman

Abstract

A bacterial staining method using fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is described for quantifying actively respiring bacteria that adhere to commercially pure titanium surfaces coated with cross-linked albumin. This has not been possible to date using ordinary DNA stains such as propidium iodide (PI) or Hoechst, both of which produce a very bright background. With this technique, it was demonstrated that the cross-linked albumin inhibited the adherence of Staphylococcus aureus and Staph. epidermidis to the titanium surface.

Citations

Jun 1, 1997·Journal of Periodontology·L RimondiniA Carrassi
Feb 14, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dénes B HorváthyZsombor Lacza
Sep 8, 1998·Journal of Biomedical Materials Research·Y H An, R J Friedman
Sep 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Y H AnR J Friedman
Nov 20, 2016·BioFactors·Dénes B HorváthyZsombor Lacza
Oct 6, 2007·The International Journal of Artificial Organs·Y QiuX Wen

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