Application of a liquid chromatographic/tandem mass spectrometric method to a urinary excretion study of rabeprazole and two of its metabolites in healthy human urine

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Chengtao LuLi Ding

Abstract

To study urinary excretion properties of rabeprazole and two of its metabolites, i.e. rabeprazole thioether and desmethyl rabeprazole thioether in human urine, a sensitive, selective, accurate and precise method for the quantification of rabeprazole and two of its metabolites using a liquid chromatographic/tandem mass spectrometric method has been developed and validated. Starting with a 200 μL urine aliquot, a general sample preparation was performed using protein precipitation with methanol. Analytes were separated on a Dikma Inspire™ C18 column (150 mm × 2.1mm, 5 μm) using a mixture of methanol and aqueous 10mM ammonium acetate buffer containing 0.05% formic acid (55:45, v/v) as mobile phase. Linearity was obtained over the concentration range of 0.1446-96.38 ng/mL, 0.3198-319.8 ng/mL and 0.05160-82.53 ng/mL for rabeprazole, rabeprazole thioether, desmethyl rabeprazole thioether in human urine, respectively. The fully validated method was applied to a urine excretion study of rabeprazole sodium administered as a 30 min intravenous infusion for the first time. The calculated cumulative urinary recovery just reached 0.04745‰, 1.272‰ and 0.1631‰ of dose within 24h post-dose for rabeprazole, rabeprazole thioether, and desmethyl ...Continue Reading

References

Jul 5, 1991·Biochemical Pharmacology·H FujisakiN Takeguchi
Mar 1, 2003·Journal of Pharmaceutical and Biomedical Analysis·Alaa El-GindyMoustafa M Maher
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·N V S RamakrishnaS Suresh Kumar
Jul 16, 2005·Rapid Communications in Mass Spectrometry : RCM·Jinchang HuangQingxiang Guo
Aug 10, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tsukasa UnoTomonori Tateishi
Jan 31, 2006·Journal of Pharmaceutical and Biomedical Analysis·Masatomo MiuraToshio Suzuki
Dec 21, 2006·International Journal of Clinical Pharmacology and Therapeutics·T SetoyamaJ Hasegawa

❮ Previous
Next ❯

Citations

Apr 12, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ashraf YousefzadehMohammad Hossein Somi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.