Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos

Theriogenology
Jingchun LiYanbing Li

Abstract

Viable sperm from sex-sorted semen without centrifugal treatment was separated by a microfluidic sperm sorter (MFSS) for IVF to improve in vitro embryo production of dairy cattle. The MFSS was originally developed to isolate motile human sperm by two laminar flows in the micro-channel (there are four chambers in an MFSS. Chamber A is the inlet for semen, chamber B is the inlet for the medium, chamber C is the exit chamber for motile sperm, and chamber D is the outlet for nonmotile sperm). Sex-sorted sperm were adjusted to 1 × 10(7) spermatozoa/mL (2 million cells/dose, sperm motility was 30% above after thawing). In a first experiment, diluted sex-sorted semen was mixed with modified Medium199(mM199) containing 5-mM caffeine for 5 minutes, resulting in variations in sperm concentration and quality parameters at chambers A, C, and D. In a second experiment, medium containing sperm from three MFSS chambers was collected and mitochondrial activity of the sperm was determined by flow cytometry, the relative activity of sperm mitochondria in chamber C (1.56 ± 0.03) was the highest in three observation areas (P < 0.05). Thus, sperm motility and mitochondrial activity of sperm was high in chamber C. In a third experiment, different co...Continue Reading

References

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Citations

May 10, 2017·Molecular Reproduction and Development·Jean M Feugang
Jan 22, 2019·The Journal of Reproduction and Development·Kohei UmezuKentaro Tanemura
Aug 11, 2020·Biomicrofluidics·Tanja HamacherLoes I Segerink
Nov 15, 2018·Micromachines·Hong-Yuan HuangDa-Jeng Yao
Aug 12, 2020·Reproduction in Domestic Animals = Zuchthygiene·Mohammed A Abdel-GhaniMasashi Nagano
Dec 12, 2020·Andrology·Kazuko OgataKenichi Yamashita
Feb 6, 2017·Biotechnology Advances·Gang Zhao, Jianping Fu

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