PMID: 8595704Dec 1, 1995Paper

Application of a modified INCSTAR Epo-trac 125/RIA for measurement of serum erythropoietin concentration in elite athletes

Clinical Biochemistry
D RobertsD J Smith

Abstract

To evaluate the analytical performance of a radioimmunoassay for measurement of erythropoietin. The INCSTAR Epo-trac 125I radioimmunoassay was examined for applications requiring sensitivity and precision within the normal range. Sensitivity was improved by increasing sample volume to 300 microL. Minimal detectable concentration was determined at 5.3 U/L, %CV ranged from 4.8-5.8 and 13.3-6.4 for intra- and inter-assay imprecision, respectively. Accuracy was maximized by controlling for lipemic and hemolyzed samples and ensuring serum was separated from the clot within 1 h of collection. Values demonstrated good correlation to the Diagnostic Systems Laboratory RIA-kit. With sample volume increased to 300 microL and control of sample preparation, the INCSTAR Epo-trac 125/RIA showed improved precision. Assay sensitivity at lower values allows resolution of changes in erythropoietin within the normal reference range. Age was not found to influence erythropoietin concentration. Within 10 days sojourn at moderate altitude an increase in circulating erythropoietin and reticulocytosis was observed in swimmers.

References

Sep 1, 1978·Endocrinology·J B Sherwood, E Goldwasser
Jan 1, 1992·European Journal of Applied Physiology and Occupational Physiology·L M WeightP Jacobs
Jan 1, 1991·European Journal of Applied Physiology and Occupational Physiology·H J SchwandtL Röcker
Jul 1, 1991·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R GareauG R Brisson
Jan 1, 1991·Thorax·J M GoldmanJ C Biggs
Oct 1, 1990·Immunology Today·J F Bazan
Apr 1, 1990·European Journal of Pediatrics·K U EckardtA Kurtz
Nov 1, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·K MatsubaraH Morii
May 1, 1989·British Journal of Haematology·L WideG Birgegård
Oct 1, 1988·British Journal of Haematology·M HellebostadP M Cotes
Jan 1, 1988·Nephron·M VedovatoM Masotti
Feb 1, 1970·Archives of Disease in Childhood·W A Marshall, J M Tanner
Jan 1, 1993·European Journal of Applied Physiology and Occupational Physiology·T KlausenH Galbo

❮ Previous
Next ❯

Citations

Jul 15, 1999·Journal of Sports Sciences·D Roberts, D J Smith
Aug 10, 2000·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·C Breymann
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·S M Donnelly, J A Miller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

European Journal of Applied Physiology and Occupational Physiology
L M WeightP Jacobs
European Journal of Applied Physiology and Occupational Physiology
T KlausenE Hippe
European Journal of Applied Physiology and Occupational Physiology
M D Koskolou, D C McKenzie
© 2021 Meta ULC. All rights reserved