Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus.

Bioprocess and Biosystems Engineering
S Lladó MaldonadoRainer Krull

Abstract

Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µmax = 0.824 1/h), Monod constant (KS = 34 × 10- 3gS/L), substrate-related biomass yield coefficient (YX/S = 0.315 gCDW/gS), and maintenance coefficient (mS = 0.0035 gS/(gCDW·h)) were determined. These parameters are now available for further studies in the field of synthetic biology.

References

Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·K Kovárová-Kovar, T Egli
Jan 3, 2001·Biotechnology and Bioengineering·Y KostovG Rao
Jul 6, 2004·Biotechnology and Bioengineering·Andrea ZanzottoKlavs F Jensen
Dec 8, 2005·Biotechnology and Bioengineering·Zhiyu ZhangKlavs F Jensen
Dec 9, 2008·Applied and Environmental Microbiology·Ralf RosensteinFriedrich Götz
Oct 15, 2010·Biotechnology Progress·Astrid EdlichRainer Krull
Jul 26, 2012·International Journal of Cell Biology·Mario MautheTassula Proikas-Cezanne
Jan 3, 2013·Biotechnology and Bioengineering·Timothy V Kirk, Nicolas Szita
May 9, 2014·Biotechnology and Bioengineering·Gena PeteratRainer Krull
Aug 2, 2016·Journal of Chemical Technology and Biotechnology·Timothy V KirkNicolas Szita
Jul 21, 2017·Lab on a Chip·Pia GruberTorsten Mayr
Oct 4, 2017·Applied Microbiology and Biotechnology·John LöfblomFriedrich Götz
Dec 26, 2017·Current Opinion in Chemical Engineering·Marco Pc Marques, Nicolas Szita
May 17, 2018·Microbial Cell Factories·Anna-Maria Marbà-ArdébolStefan Junne

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Citations

Aug 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sonali SinhaReena Rao

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