PMID: 9188819May 9, 1997Paper

Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma

Journal of Chromatography. B, Biomedical Sciences and Applications
G MaianiA Ferro-Luzzi

Abstract

We developed a method to measure plasma levels of selected polyphenols before and after ingestion of green tea. Blood samples were obtained from four healthy women before and 30 and 50 min after the ingestion of 300 ml of green tea infusion. A 1-ml volume of plasma was hydrolysed with 0.5 M HCl-methanol (1:1, v/v) for 30 min at room temperature, extracted with ethyl acetate and separated by reversed-phase chromatography. Polyphenols were identified on the basis of their retention times and by spectrum analysis. Green tea caffeine has the same retention times as caffeic acid. Consumption of green tea produces a notable increase in the plasma levels of caffeine plus caffeic acid and the appearance of measurable levels of epigallocatechingallate. In conclusion, the method was found to have the requisite features of specificity and sensitivity for monitoring plasma levels of selected tea polyphenols.

Citations

Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J H Weisburger, Fung Lung Chung
Aug 25, 2001·The British Journal of Nutrition·H CrewsG Williamson
Sep 1, 2005·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Raymond CooperDorothy M Morré
Dec 26, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jianbing WuHuizhong Wang
Feb 17, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benno F ZimmermannRudolf Galensa
Oct 24, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hsien-Tsung YaoTeng-Kuang Yeh
Sep 25, 2009·Phytotherapy Research : PTR·Łuczaj WojciechSkrzydlewska Elzbieta
Apr 7, 2004·Alcohol·Justyna OstrowskaElzbieta Skrzydlewska
Mar 4, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yoshinori MasukawaTadashi Hase
Jun 6, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·E SkrzydlewskaK Michalak
Aug 16, 2005·Chemico-biological Interactions·Izabela DobrzyńskaZbigniew Figaszewski
Oct 6, 2005·Journal of Biochemistry and Molecular Biology·Hesham A El-Beshbishy
Feb 19, 2011·Toxicology Mechanisms and Methods·A AugustyniakE Skrzydlewska
May 24, 2017·Human Molecular Genetics·Weronica E EkUNKNOWN Epigenome-Wide Association Study Consortium
Aug 29, 2003·Nature·Mauro SerafiniAlan Crozier
Apr 27, 2019·Environmental and Molecular Mutagenesis·Ion UdroiuAntonella Sgura
Aug 22, 2009·Toxicology Mechanisms and Methods·Barbara Szachowicz-PetelskaZbigniew A Figaszewski
Jul 13, 2005·International Journal of Food Sciences and Nutrition·Konstantina ArgyriMaria Kapsokefalou
Jul 20, 2002·Addiction Biology·E SkrzydlewskaR Farbiszewski
Mar 17, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K M PistersJ S Lee
Oct 24, 2007·The Journal of Nutrition·Randi L EdwardsThunder Jalili
Aug 5, 2000·The Journal of Nutrition·A Scalbert, G Williamson
Oct 21, 2020·Chemistry, an Asian Journal·Uday MaitraShruthi Sakthivel
Dec 22, 2005·Journal of Agricultural and Food Chemistry·Tomonori UnnoTakami Kakuda
Jan 30, 2007·Journal of Agricultural and Food Chemistry·Lei-Chwen LinTung-Hu Tsai
Oct 2, 2007·Journal of Agricultural and Food Chemistry·Maria de Lourdes Mata-BilbaoRosa Maria Lamuela-Raventós

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