PMID: 2830472Jan 1, 1987Paper

Application of a new medium supplement for propagation and storage of human cytomegalovirus

Microbiology and Immunology
M Okada, Y Minamishima


A new medium supplement, NU-SERUM, was evaluated for cultivation of human embryonic lung fibroblasts (HEL) and for propagation and storage of human cytomegalovirus (HCMV). NU-SERUM was comparable to fetal bovine serum (FBS) in promoting rapid growth of HEL if they were seeded at a sufficient density. HCMV replicated quite satisfactorily in HEL cultured with media supplemented with NU-SERUM as well as FBS. Inactivation of HCMV at 37 C occurred similarly when the medium contained FBS or NU-SERUM. However, at -70 C, HCMV was less stable in NU-SERUM-containing medium than in FBS-containing medium. Sorbitol added to the NU-SERUM-containing medium improved the unstableness of HCMV at -70 C, and HCMV was storable with such medium. Thus, NU-SERUM is useful as an alternative to FBS not only for growth of HEL but also for propagation and storage of HCMV.


Sep 1, 1976·Infection and Immunity·N J Schmidt, E H Lennette
Jan 1, 1985·The Journal of Cell Biology·M W Klymkowsky, D J Plummer
Feb 1, 1985·The Journal of Investigative Dermatology·R E Boissy, R Halaban
Sep 1, 1968·Journal of Virology·C Wallis, J L Melnick
Sep 1, 1983·Journal of Clinical Microbiology·C L Howell, M J Miller
Dec 1, 1984·Kidney International·P A HarperM J Karnovsky
Oct 1, 1953·The Journal of Experimental Medicine·H AMOS
Jun 14, 1962·The New England Journal of Medicine·T H WELLER, J B HANSHAW

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