Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development

Frontiers in Plant Science
Holger SpiegelRainer Fischer

Abstract

Despite decades of intensive research efforts there is currently no vaccine that provides sustained sterile immunity against malaria. In this context, a large number of targets from the different stages of the Plasmodium falciparum life cycle have been evaluated as vaccine candidates. None of these candidates has fulfilled expectations, and as long as we lack a single target that induces strain-transcending protective immune responses, combining key antigens from different life cycle stages seems to be the most promising route toward the development of efficacious malaria vaccines. After the identification of potential targets using approaches such as omics-based technology and reverse immunology, the rapid expression, purification, and characterization of these proteins, as well as the generation and analysis of fusion constructs combining different promising antigens or antigen domains before committing to expensive and time consuming clinical development, represents one of the bottlenecks in the vaccine development pipeline. The production of recombinant proteins by transient gene expression in plants is a robust and versatile alternative to cell-based microbial and eukaryotic production platforms. The transfection of plant ...Continue Reading

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Citations

Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim
Mar 31, 2017·Applied Microbiology and Biotechnology·Ingrid K Leth, Karen A McDonald
Mar 28, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Gueven EdgueMarkus Sack
May 31, 2017·Biotechnology Progress·Ingrid K Leth, Karen A McDonald
Jun 28, 2019·Frontiers in Plant Science·Stefan SchillbergMatthias Buntru
Nov 17, 2020·Frontiers in Plant Science·Mariana Fonseca XistoSérgio Oliveira de Paula

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Datasets Mentioned

BETA
AY574374

Methods Mentioned

BETA
transgenic
PCR
affinity purification
ion exchange chromatography
dot-blot
fluorescence microscopy
ELISA
transfection

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Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

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