Application of a strain-specific rRNA oligonucleotide probe targeting Pseudomonas fluorescens Ag1 in a mesocosm study of bacterial release into the environment.

Applied and Environmental Microbiology
Mette BoyeS Molin

Abstract

Sequence analysis of domains 3 and 4 of 23S rRNA from Pseudomonas fluorescens Ag1 was carried out to allow the design of a strain-specific rRNA oligonucleotide probe targeting this strain. The specificity of the probe, Ps-Ag1, was assessed by dot blot analysis and whole-cell hybridization, and it was found to be specific for P. fluorescens Ag1. The correlation between the ribosomal content of P. fluorescens Ag1 and growth rate was determined during balanced growth conditions with generation times ranging from 1.2 to 31.8 h. Hybridization of the rRNA-targeting probes combined with charged coupled device-enhanced microscopy was used to determine the rRNA content. The total RNA content per cell was determined by staining with acridine orange and charged coupled device-enhanced microscopy. After 2 h under carbon starvation conditions, the rRNA content per cell decreased to 45% of the content of an exponentially growing cell. After 1 day of carbon starvation, the rRNA content had decreased to 20%. When cells were grown at different temperatures, it was found that the rRNA content per cell was only dependent on the substrate in the temperature range from 5 to 30 degrees C. P. fluorescens Ag1 was used in a mesocosm release experiment....Continue Reading

References

Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Jan 1, 1990·Applied and Environmental Microbiology·J PützE Stackebrandt
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Jun 1, 1987·Microbiological Reviews·C R Woese
Nov 6, 1989·European Journal of Biochemistry·P HöpflN Larsen
May 1, 1988·Applied and Environmental Microbiology·D A StahlL Montgomery
Aug 1, 1982·Journal of Bacteriology·J RyalsH Bremer
Jul 1, 1993·Nucleic Acids Research·N LarsenC R Woese
Jul 1, 1956·Journal of General Microbiology·D HERBERTR C TELLING
Jul 29, 1960·Biochimica Et Biophysica Acta·F C NEIDHARDT, B MAGASANIK
Jun 1, 1988·Applied and Environmental Microbiology·J A FuhrmanA M Chan
Jan 1, 1992·Applied and Environmental Microbiology·O NybroeB Riemann
Aug 1, 1993·Applied and Environmental Microbiology·P F KempJ Laroche

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Citations

Aug 23, 2006·Critical Reviews in Biotechnology·Meltem Urgun-DemirtasKrishna Pagilla
Apr 1, 1997·Molecular Biotechnology·J K Jansson, J I Prosser
Oct 31, 1998·Applied and Environmental Microbiology·J PernthalerR Amann
May 9, 1998·Applied and Environmental Microbiology·P LebaronP Catala
Sep 15, 2000·Journal of Immunological Methods·D A VealP V Attfield
May 2, 2000·Applied and Environmental Microbiology·D B OertherL Raskin
Jul 1, 1997·Applied and Environmental Microbiology·V G MathesonL J Forney

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